Figure 6

TTF-1 plays a role in the regulation of Pax8 expression in differentiated thyroid cells. Q-PCR analysis was performed on total RNA prepared from FRTL-5 rat thyroid cells and two clones, Cl-3 and Cl-5, stable transfected with an shRNA driven against TTF-1. The expression of TTF-1, Pax8 and Tg genes was measured. For each gene, values are means ± SD of seven independent experiments in duplicate, normalized by the expression of β-actin, and expressed as percentage of the value measured in parental FRTL-5 cells. Statistical analysis uses t test.