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Table 3 Up-regulated genes after temperature increase up to 37°C (day 4) in L. infantum.

From: Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum

Clone F Log 2 F ± SD P GenBank GSS e-value Def. Id. Annotated Gene Function qRT-PCR
      Fw Rv     +/- F ± SD
Lin11D7 2.37 1.2 ± 0.1 0.004 GS598854 - 0 c LinJ31_V3.0460 Amastin, putative +  
Lin17C6 1.92 0.9 ± 0.1 0.006 GS598973 0 0 b LinJ36_V3.0640 Delta-8 fatty acid desaturase-like protein N.D.  
Lin19D1 1.88 0.9 ± 0.3 0.028 GS598855 3e-18 0 b LinJ08_V3.0680 Amastin-like protein N.D.  
         LinJ08_V3.0690 Amastin-like protein N.D.  
Lin33G2 2.29 1.2 ± 0.8 0.046 GS598857 6e-118 6e-118 b LinJ34_V3.4370 Amastin-like surface protein, putative N.D.  
Lin70F5 2.03 1.0 ± 0.4 0.045 GS598974 0 0 b LinJ36_V3.7290 Delta-8 fatty acid desaturase-like protein N.D.  
Lin77H8 2.89 1.5 ± 0.4 0.022 GS598975 3e-175 0 b LinJ08_V3.0690 Amastin-like protein N.D.  
Lin86H7 2.03 1.0 ± 0.2 0.005 GS598867 0 2e-101 b LinJ08_V3.0700 Amastin-like protein + 6.8 ± 0.9
Lin87H2 1.89 0.9 ± 0.1 0.007 GS598868 3e-15 3e-33 b LinJ08_V3.0690 Amastin-like protein N.D.  
Lin89D9 1.70 0.8 ± 0.3 0.040 GS598869 0 0 b LinJ21_V3.0770 ATP-binding cassette sub-family E, putative N.D.  
Lin90B6 1.95 1.0 ± 0.3 0.032 GS598976 0 0 a LinJ30_V3.0640 Ribosome biogenesis regulatoy protein (RRS1), putative + 1.9 ± 0.2
         LinJ30_V3.0650 Histidyl-tRNA synthetase, putative N.D.  
         LinJ30_V3.0660 Hypothetical protein, conserved N.D.  
Lin91B12 1.75 0.8 ± 0.1 0.003 GS598872 0 0 b LinJ34_V3.2660 Amastin-like surface protein N.D.  
Lin100B2 1.84 0.9 ± 0.3 0.034 GS598977 0 9e-27 b LinJ27_V3.2500 Glycosomal phosphoenolpyruvate carboxykinase N.D.  
Lin104B11 1.77 0.8 ± 0.2 0.022 GS598978 0 0 b LinJ04_V3.0570 Spermidine synthase 1, putative N.D.  
Lin104C10 1.82 0.9 ± 0.2 0.015 GS598979 0 0 b LinJ08_V3.1320 Amastin-like protein N.D.  
Lin106A1 2.43 1.3 ± 0.0 0.000 GS598980 0 0 c LinJ06_V3.1200 Hypothetical protein, conserved N.D.  
         LinJ31_V3.0590 Amino acid transporter aATP11, putative + 2.4 ± 0.3
Lin109B3 1.89 0.9 ± 0.2 0.024 GS598981 0 0 b LinJ21_V3.2130 Centromere/microtubule binding protein (cbf5), putative N.D.  
Lin113C3 2.99 1.6 ± 0.3 0.010 GS598876 3e-74 0 a LinJ14_V3.1440 Pteridine transporter + 2.5 ± 0.3
         LinJ14_V3.1450 Myo-inositol-1-phosphate synthase + 4.2 ± 0.1
Lin137A10 1.98 1.0 ± 0.3 0.039 GS598982 0 0 b LinJ24_V3.1230 Hypothetical protein, conserved N.D.  
         LinJ24_V3.1240 Translation factor SUI1, putative + 1.8 ± 0.1
Lin146E3 2.52 1.3 ± 0.3 0.043 GS598882 0 0 b LinJ31_V3.0590 Amino acid transporter aATP11, putative + 2.4 ± 0.3
Lin162E6 1.92 0.9 ± 0.3 0.044 GS598983 0 0 a LinJ14_V3.1430 Hypothetical protein, conserved N.D.  
         LinJ14_V3.1440 Pteridine transporter + 2.5 ± 0.3
         LinJ14_V3.1450 Myo-inositol-1-phosphate synthase + 4.2 ± 0.1
Lin168A2 1.87 0.9 ± 0.2 0.017 GS598984 1e-78 0 b LinJ22_V3.0670 Hypothetical protein N.D.  
         LinJ22_V3.0680 3'a2rel-related protein + 3.5 ± 0.6
Lin175D6 2.20 1.2 ± 0.4 0.023 GS598985 0 0 b LinJ31_V3.0460 Amastin, putative + 4.7 ± 1.2
Lin185A10 2.04 1.0 ± 0.3 0.036 GS598986 0 0 a LinJ28_V3.0620 MAP kinase, putative N.D.  
Lin185D7 1.75 0.0 ± 0.2 0.020 GS598987 2e-161 0 b LinJ17_V3.0200 Elongation factor 1-alpha N.D.  
Lin188H2 3.20 1.7 ± 0.6 0.042 GS598988 0 0 c LinJ08_V3.0680 Amastin-like protein N.D.  
Lin194E2 1.79 0.8 ± 0.2 0.025 GS598989 - 0 c LinJ08_V3.0710 Amastin-like protein + 6.8 ± 0.9
Lin206B6 2.08 1.0 ± 0.3 0.035 GS598990 7e-19 0 b LinJ22_V3.0680 3'a2rel-related protein + 3.5 ± 0.6
Lin207A1 1.84 0.9 ± 0.2 0.015 GS598991 0 0 b LinJ17_V3.0170 Elongation factor 1-alpha N.D.  
         LinJ17_V3.0180 Elongation factor 1-alpha N.D.  
Lin210C4 1.71 1.8 ± 0.1 0.030 GS598893 0 0 b LinJ08_V3.0690 Amastin-like protein N.D.  
Lin224G2 1.70 0.8 ± 0.2 0.014 GS598895 0 0 b LinJ08_V3.0720 Amastin-like protein N.D.  
Lin235G8 2.20 1.1 ± 0.2 0.002 GS598896 0 0 b LinJ08_V3.1320 Amastin-like protein N.D.  
Lin245E2 2.05 1.0 ± 0.3 0.032 GS598897 0 0 b LinJ22_V3.0680 3'a2rel-related protein + 3.5 ± 0.6
Lin274G6 1.84 0.9 ± 0.2 0.012 GS598992 0 0 b LinJ08_V3.0680 Amastin-like protein N.D.  
         LinJ08_V3.0690 Amastin-like protein N.D.  
Lin275A8 2.10 1.1 ± 0.1 0.003 GS598900 0 4e-168 b LinJ08_V3.0720 Amastin-like protein N.D.  
Lin282B6 2.08 1.0 ± 0.4 0.042 GS598993 0 0 b LinJ03_V3.0960 Elongation initiation factor 2 alpha subunit, putative N.D.  
Lin294A11 1.72 0.8 ± 0.1 0.001 GS598902 0 0 b LinJ08_V3.1320 Amastin-like protein N.D.  
Lin295D9 2.99 1.6 ± 0.4 0.020 GS598903 0 0 b LinJ34_V3.1720 Amastin-like surface protein, putative N.D.  
  1. This table describes clones that contain up-regulated genes under the sole influence of temperature increase (TS) that do not map with hypothetical or unknown genes. The features described are: clone number; fold change (F); base-two logarithmic scale F and standard deviation (SD) values; p-value (p); GenBank GSS accession numbers; e-values of forward (Fw) and reverse (Rv) end mappings against BLAST; clone definition (Def.) according to mapping outcomes a, b or c (see brief explanation in the text); GeneDB identifiers (Id.), the corresponding annotated gene functions; qRT-PCR results. When a given clone overlaps with more than one annotation, stage-specific regulation is only demonstrated if the qRT-PCR result is positive (+). Genes in bold are also up-regulated under TPS.