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Table 3 Up-regulated genes after temperature increase up to 37°C (day 4) in L. infantum.

From: Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum

Clone

F

Log 2 F ± SD

P

GenBank GSS

e-value

Def.

Id.

Annotated Gene Function

qRT-PCR

     

Fw

Rv

   

+/-

F ± SD

Lin11D7

2.37

1.2 ± 0.1

0.004

GS598854

-

0

c

LinJ31_V3.0460

Amastin, putative

+

 

Lin17C6

1.92

0.9 ± 0.1

0.006

GS598973

0

0

b

LinJ36_V3.0640

Delta-8 fatty acid desaturase-like protein

N.D.

 

Lin19D1

1.88

0.9 ± 0.3

0.028

GS598855

3e-18

0

b

LinJ08_V3.0680

Amastin-like protein

N.D.

 
        

LinJ08_V3.0690

Amastin-like protein

N.D.

 

Lin33G2

2.29

1.2 ± 0.8

0.046

GS598857

6e-118

6e-118

b

LinJ34_V3.4370

Amastin-like surface protein, putative

N.D.

 

Lin70F5

2.03

1.0 ± 0.4

0.045

GS598974

0

0

b

LinJ36_V3.7290

Delta-8 fatty acid desaturase-like protein

N.D.

 

Lin77H8

2.89

1.5 ± 0.4

0.022

GS598975

3e-175

0

b

LinJ08_V3.0690

Amastin-like protein

N.D.

 

Lin86H7

2.03

1.0 ± 0.2

0.005

GS598867

0

2e-101

b

LinJ08_V3.0700

Amastin-like protein

+

6.8 ± 0.9

Lin87H2

1.89

0.9 ± 0.1

0.007

GS598868

3e-15

3e-33

b

LinJ08_V3.0690

Amastin-like protein

N.D.

 

Lin89D9

1.70

0.8 ± 0.3

0.040

GS598869

0

0

b

LinJ21_V3.0770

ATP-binding cassette sub-family E, putative

N.D.

 

Lin90B6

1.95

1.0 ± 0.3

0.032

GS598976

0

0

a

LinJ30_V3.0640

Ribosome biogenesis regulatoy protein (RRS1), putative

+

1.9 ± 0.2

        

LinJ30_V3.0650

Histidyl-tRNA synthetase, putative

N.D.

 
        

LinJ30_V3.0660

Hypothetical protein, conserved

N.D.

 

Lin91B12

1.75

0.8 ± 0.1

0.003

GS598872

0

0

b

LinJ34_V3.2660

Amastin-like surface protein

N.D.

 

Lin100B2

1.84

0.9 ± 0.3

0.034

GS598977

0

9e-27

b

LinJ27_V3.2500

Glycosomal phosphoenolpyruvate carboxykinase

N.D.

 

Lin104B11

1.77

0.8 ± 0.2

0.022

GS598978

0

0

b

LinJ04_V3.0570

Spermidine synthase 1, putative

N.D.

 

Lin104C10

1.82

0.9 ± 0.2

0.015

GS598979

0

0

b

LinJ08_V3.1320

Amastin-like protein

N.D.

 

Lin106A1

2.43

1.3 ± 0.0

0.000

GS598980

0

0

c

LinJ06_V3.1200

Hypothetical protein, conserved

N.D.

 
        

LinJ31_V3.0590

Amino acid transporter aATP11, putative

+

2.4 ± 0.3

Lin109B3

1.89

0.9 ± 0.2

0.024

GS598981

0

0

b

LinJ21_V3.2130

Centromere/microtubule binding protein (cbf5), putative

N.D.

 

Lin113C3

2.99

1.6 ± 0.3

0.010

GS598876

3e-74

0

a

LinJ14_V3.1440

Pteridine transporter

+

2.5 ± 0.3

        

LinJ14_V3.1450

Myo-inositol-1-phosphate synthase

+

4.2 ± 0.1

Lin137A10

1.98

1.0 ± 0.3

0.039

GS598982

0

0

b

LinJ24_V3.1230

Hypothetical protein, conserved

N.D.

 
        

LinJ24_V3.1240

Translation factor SUI1, putative

+

1.8 ± 0.1

Lin146E3

2.52

1.3 ± 0.3

0.043

GS598882

0

0

b

LinJ31_V3.0590

Amino acid transporter aATP11, putative

+

2.4 ± 0.3

Lin162E6

1.92

0.9 ± 0.3

0.044

GS598983

0

0

a

LinJ14_V3.1430

Hypothetical protein, conserved

N.D.

 
        

LinJ14_V3.1440

Pteridine transporter

+

2.5 ± 0.3

        

LinJ14_V3.1450

Myo-inositol-1-phosphate synthase

+

4.2 ± 0.1

Lin168A2

1.87

0.9 ± 0.2

0.017

GS598984

1e-78

0

b

LinJ22_V3.0670

Hypothetical protein

N.D.

 
        

LinJ22_V3.0680

3'a2rel-related protein

+

3.5 ± 0.6

Lin175D6

2.20

1.2 ± 0.4

0.023

GS598985

0

0

b

LinJ31_V3.0460

Amastin, putative

+

4.7 ± 1.2

Lin185A10

2.04

1.0 ± 0.3

0.036

GS598986

0

0

a

LinJ28_V3.0620

MAP kinase, putative

N.D.

 

Lin185D7

1.75

0.0 ± 0.2

0.020

GS598987

2e-161

0

b

LinJ17_V3.0200

Elongation factor 1-alpha

N.D.

 

Lin188H2

3.20

1.7 ± 0.6

0.042

GS598988

0

0

c

LinJ08_V3.0680

Amastin-like protein

N.D.

 

Lin194E2

1.79

0.8 ± 0.2

0.025

GS598989

-

0

c

LinJ08_V3.0710

Amastin-like protein

+

6.8 ± 0.9

Lin206B6

2.08

1.0 ± 0.3

0.035

GS598990

7e-19

0

b

LinJ22_V3.0680

3'a2rel-related protein

+

3.5 ± 0.6

Lin207A1

1.84

0.9 ± 0.2

0.015

GS598991

0

0

b

LinJ17_V3.0170

Elongation factor 1-alpha

N.D.

 
        

LinJ17_V3.0180

Elongation factor 1-alpha

N.D.

 

Lin210C4

1.71

1.8 ± 0.1

0.030

GS598893

0

0

b

LinJ08_V3.0690

Amastin-like protein

N.D.

 

Lin224G2

1.70

0.8 ± 0.2

0.014

GS598895

0

0

b

LinJ08_V3.0720

Amastin-like protein

N.D.

 

Lin235G8

2.20

1.1 ± 0.2

0.002

GS598896

0

0

b

LinJ08_V3.1320

Amastin-like protein

N.D.

 

Lin245E2

2.05

1.0 ± 0.3

0.032

GS598897

0

0

b

LinJ22_V3.0680

3'a2rel-related protein

+

3.5 ± 0.6

Lin274G6

1.84

0.9 ± 0.2

0.012

GS598992

0

0

b

LinJ08_V3.0680

Amastin-like protein

N.D.

 
        

LinJ08_V3.0690

Amastin-like protein

N.D.

 

Lin275A8

2.10

1.1 ± 0.1

0.003

GS598900

0

4e-168

b

LinJ08_V3.0720

Amastin-like protein

N.D.

 

Lin282B6

2.08

1.0 ± 0.4

0.042

GS598993

0

0

b

LinJ03_V3.0960

Elongation initiation factor 2 alpha subunit, putative

N.D.

 

Lin294A11

1.72

0.8 ± 0.1

0.001

GS598902

0

0

b

LinJ08_V3.1320

Amastin-like protein

N.D.

 

Lin295D9

2.99

1.6 ± 0.4

0.020

GS598903

0

0

b

LinJ34_V3.1720

Amastin-like surface protein, putative

N.D.

 
  1. This table describes clones that contain up-regulated genes under the sole influence of temperature increase (TS) that do not map with hypothetical or unknown genes. The features described are: clone number; fold change (F); base-two logarithmic scale F and standard deviation (SD) values; p-value (p); GenBank GSS accession numbers; e-values of forward (Fw) and reverse (Rv) end mappings against BLAST; clone definition (Def.) according to mapping outcomes a, b or c (see brief explanation in the text); GeneDB identifiers (Id.), the corresponding annotated gene functions; qRT-PCR results. When a given clone overlaps with more than one annotation, stage-specific regulation is only demonstrated if the qRT-PCR result is positive (+). Genes in bold are also up-regulated under TPS.
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BMC Genomics

ISSN: 1471-2164

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