Skip to main content

Table 4 Down-regulated genes after temperature increase up to 37°C (day 4) in L. infantum.

From: Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum

Clone F Log 2 F ± SD P GenBank GSS e-value Def. Id. Annotated Gene Function qRT-PCR
      Fw Rv     +/- F ± SD
Lin9E5 -1.77 -0.8 ± 0.3 0.033 GS598994 4e-131 0 b LinJ35_V3.1150 Oligosaccharyl transferase-like protein N.D.  
Lin40G12 -1.97 -1.0 ± 0.2 0.008 GS598916 2e-161 0 b LinJ23_V3.1550 Hypothetical protein, unknown function N.D.  
         LinJ23_V3.1560 Lathosterol oxidase-like protein + 5.0 ± 0.7
Lin49B7 -4.38 -2.1 ± 0.0 0.000 GS598995 0 4e-64 a LinJ34_V3.4160 Phosphatidylinositol-3-kinase (tor2)-like protein N.D.  
Lin 60B1 -2.41 -1.3 ± 0.4 0.025 GS598996 0 4e-162 c LinJ36_V3.7040 Hypothetical protein, conserved N.D.  
         LinJ31_V3.2370 3'-nucleotidase/nuclease, putative + 7.2 ± 1.0
Lin63F3 -2.23 -1.2 ± 0.4 0.043 GS598997 0 0 a LinJ36_V3.6550 Glucose transporter lmgt2, putative + 6.1 ± 0.8
         LinJ36_V3.6560 Glucose transporter, putative + 6.1 ± 0.8
Lin84E8 -2.26 -1.2 ± 0.2 0.007 GS598998 0 0 a LinJ31_V3.2370 3'-nucleotidase/nuclease, putative + 7.2 ± 1.0
         LinJ31_V3.2380 3'-nucleotidase/nuclease precursor, putative + 7.2 ± 1.0
Lin85H1 -1.74 -0.8 ± 0.2 0.025 GS598999 1e-57 1e-20 b LinJ30_V3.3440 CAS/CSE importin domain protein, putative N.D.  
Lin93H3 -2.26 -1.2 ± 04 0.030 GS599000 0 0 a LinJ31_V3.2370 3'-nucleotidase/nuclease, putative + 7.2 ± 1.0
         LinJ31_V3.2380 3'-nucleotidase/nuclease precursor, putative + 7.2 ± 1.0
Lin97D1 -3.41 -1.7 ± 0.3 0.001 GS598931 0 0 a LinJ06_V3.1320 Pteridine transporter, putative + 2.3 ± 0.3
Lin98G10 -2.13 -1.1 ± 0.2 0.015 GS599001 0 0 b LinJ30_V3.2770 Hypothetical protein, conserved N.D.  
         LinJ30_V3.2780 Superoxide dismutase, putative + 3.7 ± 0.0
Lin111C2 -2.74 -1.5 ± 0.1 0.002 GS599002 0 0 a LinJ09_V3.0650 Serine peptidase family S51, peptidase E, putative N.D.  
Lin150E4 -1.86 -0.9 ± 0.3 0.036 GS599003 0 8e-22 b LinJ13_V3.1060 Calmodulin, putative N.D.  
Lin155H12 -2.34 -1.2 ± 0.3 0.018 GS599004 0 0 a LinJ36_V3.0250 Peptidyl-prolyl cis-trans isomerase, putative N.D.  
Lin157D8 -2.27 -1.2 ± 0.1 0.002 GS599005 0 - c LinJ31_V3.2380 3'-nucleotidase/nuclease precursor, putative + 7.2 ± 1.0
Lin179B4 -1.76 -0.8 ± 0.1 0.004 GS599006 0 0 b LinJ07_V3.0030
LinJ07_V3.0040
LinJ07_V3.0050
LinJ07_V3.0060
Hypothetical protein, conserved
Hypothetical protein, conserved
Hypothetical protein, conserved
N.D.
N.D.
N.D.
+
 
  Alpha-adaptin-like protein   5.3 ± 0.4
Lin182F2 -2.23 -1.2 ± 0.1 0.008 GS598952 0 0 b LinJ25_V3.0740 Eukaryotic initiation factor 5a, putative N.D.  
         LinJ25_V3.0750 Eukaryotic initiation factor 5a, putative N.D.  
Lin187C10 -4.72 -2.2 ± 0.4 0.013 GS598953 0 0 b LinJ06_V3.1320 Pteridine transporter, putative + 2.3 ± 0.3
Lin204A11 -1.76 -0.8 ± 0.3 0.038 GS599007 - 1e-165 c LinJ09_V3.0650 Serine peptidase, family S51, peptidase E, putative N.D.  
Lin210B7 -1.74 -0.8 ± 0.2 0.016 GS599008 0 0 a LinJ32_V3.3690
LinJ32_V3.3700
DEAD/DEAH box helicase, putative +
N.D.
3.3 ± 0.8
  Hypothetical protein, conserved   
Lin229E6 -3.30 -1.7 ± 0.3 0.005 GS598963 0 0 a LinJ06_V3.1320
LinJ06_V3.1330
Pteridine transporter, putative
Coproporphyrinogen III oxidase, putative
+
+
2.3 ± 0.3
4.5 ± 0.6
Lin242E2 -2.37 -1.2 ± 0.4 0.039 GS599009 1e-137 0 a LinJ31_V3.2370
LinJ31_V3.2380
3'-nucleotidase/nuclease, putative
3'-nucleotidase/nuclease precursor, putative
+
+
7.2 ± 1.0
7.2 ± 1.0
Lin255E12 -2.54 -1.3 ± 0.3 0.011 GS599010 0 0 b LinJ28_V3.0210 Histone H2B variant N.D.  
  1. This table contains clones that map against down-regulated genes (not hypothetical or unknown) with the single effect of temperature increase (TS). The features described are: clone number; F; base-two logarithmic scale F and SD values; p; GenBank GSS accession numbers; e-values; Def. according to mapping outcomes a, b or c (see brief explanation in the text); Id.; annotated gene function; qRT-PCR. When a given clone overlaps with more than one annotation, stage-specific regulation is only demonstrated if the qRT-PCR result is positive (+). Genes in bold are also down-regulated under TPS.