Skip to main content

Table 5 Differentially regulated genes after pH4.5 treatment (day 4) in L. infantum.

From: Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum

Clone F Log 2 F ± SD p GenBank
GSS
e-value Def. Id. Annotated Gene Function qRT-PCR
      Fw Rv     +/- F ± SD
Lin9E8 2.03 1.0 ± 0.1 0.003 GS599011 0 0 a LinJ24_V3.0020 Clathin coat assembly protein, putative + 7.6 ± 0.4
         LinJ24_V3.0030 Hypothetical protein, conserved N.D.  
         LinJ24_V3.0040 60S ribosomal protein L17, putative N.D.  
Lin10H12 2.26 1.2 ± 0.1 0.001 GS599012 0 0 a LinJ31_V3.0860 Triacylglycerol lipase-like protein N.D.  
         LinJ31_V3.0870 Lipase precursor-like protein N.D.  
Lin21H10 2.46 1.3 ± 0.1 0.001 GS598910 0 0 b LinJ26_v3.1670 Sphingolipid delta-4 desaturase, putative N.D.  
Lin33G5 1.76 0.8 ± 0.0 0.000 GS599013     LinJ27_V3.1300 60S acidic ribosomal protein, putative N.D.  
Lin37C10 2.74 1.5 ± 0.2 0.006 GS599014 0 0 b LinJ33_V3.0280 RNA binding protein rggm, putative N.D.  
Lin58C1 2.32 1.2 ± 0.2 0.001 GS599015 0 0 b LinJ22_V3.1360 Hypothetical protein, unknown fuction N.D.  
         LinJ22_V3.1370 60S ribosomal protein L14 N.D.  
         LinJ22_V3.1380 Dephospho-CoA kinase, putative + -2.9 ± 0.3
Lin63B7 1.83 0.9 ± 0.1 0.002 GS599016 1e-100 1e-103 b LinJ15_V3.1200 60S acidic ribosomal protein P2 N.D.  
Lin66A8 2.28 1.2 ± 0.1 0.006 GS599017 0 0 a LinJ22_V3.0470 Hypothetical protein, conserved N.D.  
         LinJ22_V3.0480 Ubiquitin-conjugating enzyme-like protein + -3.1 ± 0.8
Lin80C3 3.15 1.7 ± 0.3 0.004 GS599018 0 0 b LinJ28_V3.3250 Glucose-6-phosphate N-acetyltransferase N.D.  
Lin 95F10 2.26 1.2 ± 0.1 0.002 GS599019 0 0 a LinJ28_V3.2360 Ribosomal protein S29, putative N.D.  
Lin100F8 2.12 1.1 ± 0.2 0.007 GS599020 0 2e-161 b LinJ35_V3.3330 60S ribosomal protein L31, putative N.D.  
         LinJ35_V3.3340 60S ribosomal protein L31, putative N.D.  
Lin107C12 2.90 1.5 ± 0.2 0.001 GS599021 7e-130 5e-134 a LinJ11_V3.1180 40S ribosomal protein S15a, putative N.D.  
Lin122C5 1.93 0.9 ± 0.1 0.005 GS599022 0 0 b LinJ30_V3.3770 CPSF-domain protein, putative N.D.  
         LinJ30_V3.3780 60S acidic ribosomal protein P2, putative N.D.  
         LinJ30_V3.3790 60S acidic ribosomal protein P2, putative N.D.  
Lin135F6 2.63 1.4 ± 0.3 0.036 GS599023 0 0 b LinJ29_V3.1920 40S ribosomal protein S15a, putative N.D.  
         LinJ29_V3.1930 Hypothetical protein, conserved N.D.  
Lin137A10 2.00 1.0 ± 0.2 0.019 GS599024 0 0 b LinJ24_V3.1230 Hypothetical protein, conserved N.D.  
         LinJ24_V3.1240 Translation factor SUI1, putative + -5.0 ± 0.6
Lin144F11 1.72 0.8 ± 0.2 0.032 GS599025 0 0 a LinJ31_V3.2210 Prostaglandin F2α synthetase N.D.  
Lin161C9 2.54 1.3 ± 0.1 0.003 GS599026 0 1e-177 b LinJ26_V3.1670 Sphingolipid delta-4 desaturase, putative N.D.  
Lin162A9 1.97 1.0 ± 0.2 0.024 GS599027 0 0 b LinJ22_V3.0470 Hypothetical protein, conserved N.D.  
         LinJ22_V3.0480 Ubiquitin-conjugating enzyme-like protein + -3.1 ± 0.8
Lin182F2 3.27 1.7 ± 0.2 0.009 GS599028 0 0 b LinJ25_V3.0740 Eukaryotic initiation factor 5a, putative N.D.  
         LinJ25_V3.0750 Eukaryotic initiation factor 5a, putative N.D.  
Lin200H12 2.54 1.3 ± 0.1 0.005 GS599029 0 0 a LinJ14_V3.1340 Hypothetical protein, unknown funcion N.D.  
         LinJ14_V3. 1350 Ubiquitin/ribosomal protein S27a, putative + -4.8 ± 0.4
         LinJ14_V3.1360 Hypothetical protein, conserved N.D.  
Lin218E3 1.82 0.9 ± 0.1 0.001 GS599030 0 0 b LinJ31_V3.2210 Prostaglandin F2α synthase N.D.  
Lin247D7 2.41 1.3 ± 0.4 0.018 GS599031 5e-109 0 a LinJ28_V3.0090 Adenylate cyclase-like protein + -3.5 ± 0.6
         LinJ28_V3.0100 Hypothetical protein, conserved N.D.  
         LinJ28_V3.0110 Proteasome beta 3 subunit, putative N.D.  
Lin254A4 1.93 0.9 ± 0.2 0.009 GS599032 0 0 b LinJ04_V3.1250 Actin N.D.  
Lin254H7 1.73 0.8 ± 0.1 0.004 GS599033 0 0 b LinJ04_V3.1250 Actin N.D.  
Lin261F8 2.84 1.5 ± 0.2 0.007 GS599034 0 0 b LinJ21_V3.1310 40S ribosomal protein S23, putative N.D.  
Lin266F6 1.79 0.8 ± 0.1 0.009 GS599035 0 0 b LinJ27_V3.0300 Acyl carrier protein, putative N.D.  
Lin267B9 1.74 0.8 ± 0.2 0.010 GS599036 0 0 b LinJ36_V3.0580 Hypothetical protein, conserved N.D.  
         LinJ36_V3.0590 Ubiquitin-like protein, putative + -2.3 ± 0.0
         LinJ36_V3.0600 Cdc2-related kinase N.D.  
Lin269B5 2.75 1.5 ± 0.2 0.002 GS599037 0 0 b LinJ29_V3.2970 40S ribosomal protein S19-like protein N.D.  
Lin282B6 2.44 1.3 ± 0.2 0.009 GS599038 0 0 a LinJ03_V3.0960 Elongation initiation factor 2α subunit, putative N.D.  
Lin290G8 1.80 0.8 ± 0.1 0.003 GS599039 0 0 a LinJ17_V3.1520 Otubain cysteine peptidase, Clan CA. family C65, putative N.D.  
Lin43G10 -5.36 -2.4 ± 0.3 0.007 GS599040 0 0 c LinJ28_V3.3060 Heat-shock protein hsp70, putative + -2.1 ± 0.2
Lin130C5 -4.71 -2.2 ± 0.3 0.041 GS599041 0 0 b LinJ36_V3.3170 Exosome complex exonuclease RRP41, putative N.D.  
         LinJ36_V3.3180 Clathrin coat assembly protein-like protein N.D.  
         LinJ36_V3.3190 Pre-mRNA branch-site protein p14 + -4.7 ± 2.3
         LinJ36_V3.3200 Hypothetical protein, conserved N.D.  
Lin173E11 -7.74 -3.0 ± 0.4 0.002 GS599042 6e-44 3e-147 b LinJ36_V3.2280 ER-golgi transport protein erv25 precursor, putative N.D.  
Lin177H3 -5.08 -2.3 ± 0.2 0.001 GS599043 0 4e-60 b LinJ28_V3.3060 Heat shock protein hsp70, putative + -2.1 ± 0.2
Lin197E1 -2.53 -1.3 ± 0.1 0.007 GS599044 0 0 c LinJ18_V3.0830 Periodic tryptophan protein 2-like protein - 1.4 ± 0.4
         LinJ23_V3.1610 Acetyltransferase-like protein + -2.1 ± 0.2
Lin228H5 -7.90 -3.0 ± 0.4 0.012 GS599045 7e-196 0 b LinJ21_V3.0310 Hexokinase, putative N.D.  
Lin281H8 -2.01 -1.0 ± 0.1 0.001 GS599046 8e-136 2e-102 b LinJ35_V3.1580 Metacaspase, putative N.D.  
Lihsp70 -4.21 -2.0 ± 0.2 0.004 XM001470292 - - - - L. infantum hsp70 - DNA microarray control spot + -2.1 ± 0.2
Ldohsp70 -4.57 -2.2 ± 0.1 0.028 - - - - - L. donovani hsp70 - DNA microarray control spot N.D.  
Lmahsp70 -3.85 -1.9 ± 0.2 0.017 - - - - - L. major hsp70 -DNA microarray control spot N.D.  
  1. This table contains clones that map against up- and down-regulated genes (not hypothetical or unknown) under pH decrease (PS). The features described are: clone number; F; base-two logarithmic scale F and SD values; p; GenBank GSS accession numbers; e-values; Def. according to mapping outcomes a, b or c (see brief explanation in the text); Id.; annotated gene function; qRT-PCR. When a given clone overlaps with more than one annotation, stage-specific regulation is only demonstrated if the qRT-PCR result is positive (+). Genes in bold are also up-regulated under TPS.