Figure 1

Northern blots verify the presence of Xcc sRNAs. RNA samples isolated from exponential phase cells cultured in NYG, XVM2 and MMX medium were analyzed by Northern blotting using DNA probes complementary to cDNA clones of the sRNA candidates (for more detail see Methods). A to G represent the Northern blotting results using DNA probes complementary to cDNA clones of the sRNA candidates sRNA-C1 to sRNA-C7, respectively. Transcript sizes are approximate and compared to RiboRulerTM RNA ladder Low Range (Fermentas) that labeled by Turbo LabelingTM Kit (KPL) (M). Corresponding ethidium bromide stained gels show equal loading of total RNA in all lanes.