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Table 1 Differentially regulated proteins in A. fumigatus after a temperature shift from 30°C to 48°C.

From: Integrative analysis of the heat shock response in Aspergillus fumigatus

Protein T = 30 T = 60 T = 120 T = 180 Osc
Up regulated      
Chaperone      
AFUA_1G07440 (molecular chaperone Hsp70) 1.95 2.45 2.06 2.45 No
AFUA_1G11180 (heat shock protein/chaperonin HSP78) 1.18 1.24 0.77 1.33 No
AFUA_1G12610 (HSP 70 chaperone HSP88) 1.19 1.06 0.59 1.34 Yes
AFUA_2G04620 (Hsp70 chaperone BiP/Kar2) 0.22 0.84 1.50 1.68 No
AFUA_2G09290 (antigenic mitochondrial protein HSP60) 0.43 1.28 2.17 1.88 No
AFUA_2G09960 (mitochondrial Hsp70 chaperone (Ssc70)) 0.18 0.81 1.47 1.23 No
AFUA_3G14540 (heat shock protein Hsp30/Hsp42) -0.24 1.23 2.37 2.35 No
AFUA_4G10010 (Hsp90 co-chaperone Cdc37) 1.13 1.22 1.84 1.97 No
AFUA_4G12850 (calnexin) 0.19 0.59 1.84 1.58 No
AFUA_5G04170 (molecular chaperone and allergen Mod-E/Hsp90/Hsp1) 3.11 3.68 3.13 3.76 No
AFUA_5G07340 (DnaJ domain protein Psi) 0.50 1.01 2.31 1.58 No
AFUA_7G01860 (heat shock protein (Sti1)) 0.38 1.30 1.92 1.70 No
Cell wall and cytoskeleton      
AFUA_1G02550 (tubulin alpha-1 subunit) 1.04 0.92 -0.19 0.48 Yes
AFUA_2G07420 (actin-bundling protein Sac6) 1.48 1.27 0.30 1.16 Yes
Transport      
AFUA_6G07120 (nuclear movement protein NudC) -0.64 0.60 1.79 1.18 Yes
Energy generation      
AFUA_2G13240 (V-type ATPase, B subunit) 0.95 0.66 0.56 1.02 No
Defence against oxidative and nitrosative stress      
AFUA_4G03410 (flavohemoprotein) 0.73 0.90 0.97 1.04 No
AFUA_4G09110 (cytochrome c peroxidase Ccp1) 0.00 1.29 1.92 2.03 No
AFUA_5G09910 (nitroreductase family protein) -1.18 -0.53 1.02 1.32 No
AFUA_6G02280 (allergen Asp F3) -0.54 0.35 1.62 1.13 Yes
Signal transduction      
AFUA_1G05610 (protein phosphatase 2a 65 kd regulatory subunit) 1.47 1.47 0.98 1.27 No
AFUA_4G12450 (conserved lysine-rich protein) -0.01 0.21 1.34 1.09 No
Carbohydrate metabolism      
AFUA_1G10350 (phosphoglycerate kinase PgkA) 0.12 0.42 1.08 0.80 No
AFUA_1G13500 (transketolase TktA) 1.07 0.54 -0.62 -0.01 Yes
AFUA_2G05910 (hexokinase Kxk) 1.39 1.27 0.35 0.73 Yes
AFUA_2G10660 (mannitol-1-phosphate dehydrogenase) 1.70 1.67 1.09 1.46 No
AFUA_3G08660 (isocitrate dehydrogenase Idp1) 1.19 0.95 0.08 0.42 Yes
AFUA_4G04680 (FGGY-family carbohydrate kinase) 1.12 0.88 0.29 0.79 Yes
AFUA_4G11080 (acetyl-coenzyme A synthetase FacA) 1.64 1.27 -0.10 0.88 Yes
AFUA_4G11650 (alpha-ketoglutarate dehydrogenase complex subunit Kgd1) 1.72 1.39 0.49 0.95 Yes
AFUA_6G04210 (mannosyl-oligosaccharide glucosidase) 1.29 1.21 0.77 0.97 No
AFUA_6G04920 (NAD-dependent formate dehydrogenase AciA/Fdh) 1.17 0.66 -0.67 0.05 Yes
AFUA_6G08050 (6-phosphogluconate dehydrogenase Gnd1) 2.21 1.85 0.55 1.03 Yes
AFUA_6G10650 (ATP citrate lyase, subunit 1) 1.38 0.92 -0.16 0.33 Yes
Nitrogen metabolism      
AFUA_1G10130 (adenosylhomocysteinase) 1.88 1.46 0.05 0.60 Yes
AFUA_1G12840 (nitrite reductase NiiA) 2.75 2.30 1.19 1.54 Yes
AFUA_2G11260 (3-isopropylmalate dehydratase) 1.18 0.90 -0.09 0.44 Yes
AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D) 1.53 0.66 -1.08 -0.08 Yes
AFUA_4G07710 (pyruvate carboxylase) 2.29 1.84 0.78 1.25 Yes
AFUA_4G10460 (homocitrate synthase) 1.56 1.04 0.31 0.88 Yes
AFUA_4G13120 (glutamine synthetase) 0.67 1.48 1.72 1.84 No
Ubiquitination and proteolysis      
AFUA_4G09030 (aminopeptidase) 1.58 1.31 0.51 1.30 Yes
AFUA_5G04330 (aminopeptidase) 1.07 1.08 0.07 0.35 Yes
Protein biosynthesis/Translation      
AFUA_1G02030 (eukaryotic translation initiation factor 3 subunit EifCb) 1.33 0.64 -0.10 0.43 Yes
AFUA_1G06390 (translation elongation factor EF-1 alpha subunit) 2.00 1.28 -0.51 0.69 Yes
AFUA_2G13530 (translation elongation factor EF-2 subunit) 1.88 1.48 -0.43 0.62 Yes
AFUA_3G08160 (eukaryotic translation initiation factor 4) 1.81 1.48 0.84 1.18 Yes
AFUA_4G12920 (histidyl-tRNA synthetase, mitochondrial precursor) 1.10 1.20 0.64 1.18 Yes
AFUA_5G05920 (glycyl-tRNA synthetase) 1.56 1.26 0.23 0.83 Yes
AFUA_6G04570 (translation elongation factor eEF-1 subunit gamma) 1.20 1.10 0.22 0.71 Yes
AFUA_7G05660 (translation elongation factor eEF-3) 2.02 1.43 -0.19 0.70 Yes
AFUA_8G03880 (alanyl-tRNA synthetase) 1.37 0.80 -0.22 0.32 Yes
Transcription      
AFUA_1G15620 (DEAD box RNA helicase HelA) 1.43 1.40 0.24 0.70 Yes
Cell cycle      
AFUA_2G17110 (cell division control protein Cdc48) 1.01 1.11 0.50 0.94 Yes
Down regulated      
Chaperone      
AFUA_2G13040 (mitochondrial co-chaperone GrpE) -1.12 -0.73 0.21 -0.57 Yes
Transport      
AFUA_5G03690 (CRAL/TRIO domain protein) -1.29 -0.61 0.37 -0.53 Yes
Energy generation      
AFUA_2G13010 (cytochrome c oxidase polypeptide vib) -1.79 -1.54 -0.12 -1.12 Yes
Carbohydrate metabolism      
AFUA_1G09930 (glycerol dehydrogenase Gcy1) -1.16 -1.44 -1.25 -0.98 No
AFUA_3G09230 (carboxylesterase) 0.14 -0.69 -1.07 -1.07 No
Nitrogen metabolism      
AFUA_3G09320 (serine hydroxymethyltransferase) 0.06 -0.67 -1.01 -1.12 No
Protein biosynthesis/Translation      
AFUA_6G02750 (nascent polypeptide-associated complex (NAC) subunit) -1.35 -1.21 -0.85 -1.36 No
AFUA_6G12660 (40S ribosomal protein S10b) -1.35 -1.21 -0.85 -1.36 No
Transcription      
AFUA_3G08580 (glycine-rich RNA-binding protein) -1.27 -1.18 -0.84 -2.09 No
AFUA_8G05300 (RNA polymerase II subunit 7) -1.38 -0.97 -0.72 -1.81 No
  1. Relative protein abundance is depicted as log2-ratio. Logarithmized normalized intensities of the respective time points were subtracted by logarithmized normalized intensity of time point t = 0. Osc describes whether the protein is oscillatingly regulated.