Table 1 Differentially regulated proteins in A. fumigatus after a temperature shift from 30°C to 48°C.

Up regulated

Chaperone

AFUA_1G07440 (molecular chaperone Hsp70)

1.95

2.45

2.06

2.45

No

AFUA_1G11180 (heat shock protein/chaperonin HSP78)

1.18

1.24

0.77

1.33

No

AFUA_1G12610 (HSP 70 chaperone HSP88)

1.19

1.06

0.59

1.34

Yes

AFUA_2G04620 (Hsp70 chaperone BiP/Kar2)

0.22

0.84

1.50

1.68

No

AFUA_2G09290 (antigenic mitochondrial protein HSP60)

0.43

1.28

2.17

1.88

No

AFUA_2G09960 (mitochondrial Hsp70 chaperone (Ssc70))

0.18

0.81

1.47

1.23

No

AFUA_3G14540 (heat shock protein Hsp30/Hsp42)

-0.24

1.23

2.37

2.35

No

AFUA_4G10010 (Hsp90 co-chaperone Cdc37)

1.13

1.22

1.84

1.97

No

AFUA_4G12850 (calnexin)

0.19

0.59

1.84

1.58

No

AFUA_5G04170 (molecular chaperone and allergen Mod-E/Hsp90/Hsp1)

3.11

3.68

3.13

3.76

No

AFUA_5G07340 (DnaJ domain protein Psi)

0.50

1.01

2.31

1.58

No

AFUA_7G01860 (heat shock protein (Sti1))

0.38

1.30

1.92

1.70

No

Cell wall and cytoskeleton

AFUA_1G02550 (tubulin alpha-1 subunit)

1.04

0.92

-0.19

0.48

Yes

AFUA_2G07420 (actin-bundling protein Sac6)

1.48

1.27

0.30

1.16

Yes

Transport

AFUA_6G07120 (nuclear movement protein NudC)

-0.64

0.60

1.79

1.18

Yes

Energy generation

AFUA_2G13240 (V-type ATPase, B subunit)

0.95

0.66

0.56

1.02

No

Defence against oxidative and nitrosative stress

AFUA_4G03410 (flavohemoprotein)

0.73

0.90

0.97

1.04

No

AFUA_4G09110 (cytochrome c peroxidase Ccp1)

0.00

1.29

1.92

2.03

No

AFUA_5G09910 (nitroreductase family protein)

-1.18

-0.53

1.02

1.32

No

AFUA_6G02280 (allergen Asp F3)

-0.54

0.35

1.62

1.13

Yes

Signal transduction

AFUA_1G05610 (protein phosphatase 2a 65 kd regulatory subunit)

1.47

1.47

0.98

1.27

No

AFUA_4G12450 (conserved lysine-rich protein)

-0.01

0.21

1.34

1.09

No

Carbohydrate metabolism

AFUA_1G10350 (phosphoglycerate kinase PgkA)

0.12

0.42

1.08

0.80

No

AFUA_1G13500 (transketolase TktA)

1.07

0.54

-0.62

-0.01

Yes

AFUA_2G05910 (hexokinase Kxk)

1.39

1.27

0.35

0.73

Yes

AFUA_2G10660 (mannitol-1-phosphate dehydrogenase)

1.70

1.67

1.09

1.46

No

AFUA_3G08660 (isocitrate dehydrogenase Idp1)

1.19

0.95

0.08

0.42

Yes

AFUA_4G04680 (FGGY-family carbohydrate kinase)

1.12

0.88

0.29

0.79

Yes

AFUA_4G11080 (acetyl-coenzyme A synthetase FacA)

1.64

1.27

-0.10

0.88

Yes

AFUA_4G11650 (alpha-ketoglutarate dehydrogenase complex subunit Kgd1)

1.72

1.39

0.49

0.95

Yes

AFUA_6G04210 (mannosyl-oligosaccharide glucosidase)

1.29

1.21

0.77

0.97

No

AFUA_6G04920 (NAD-dependent formate dehydrogenase AciA/Fdh)

1.17

0.66

-0.67

0.05

Yes

AFUA_6G08050 (6-phosphogluconate dehydrogenase Gnd1)

2.21

1.85

0.55

1.03

Yes

AFUA_6G10650 (ATP citrate lyase, subunit 1)

1.38

0.92

-0.16

0.33

Yes

Nitrogen metabolism

AFUA_1G10130 (adenosylhomocysteinase)

1.88

1.46

0.05

0.60

Yes

AFUA_1G12840 (nitrite reductase NiiA)

2.75

2.30

1.19

1.54

Yes

AFUA_2G11260 (3-isopropylmalate dehydratase)

1.18

0.90

-0.09

0.44

Yes

AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D)

1.53

0.66

-1.08

-0.08

Yes

AFUA_4G07710 (pyruvate carboxylase)

2.29

1.84

0.78

1.25

Yes

AFUA_4G10460 (homocitrate synthase)

1.56

1.04

0.31

0.88

Yes

AFUA_4G13120 (glutamine synthetase)

0.67

1.48

1.72

1.84

No

Ubiquitination and proteolysis

AFUA_4G09030 (aminopeptidase)

1.58

1.31

0.51

1.30

Yes

AFUA_5G04330 (aminopeptidase)

1.07

1.08

0.07

0.35

Yes

Protein biosynthesis/Translation

AFUA_1G02030 (eukaryotic translation initiation factor 3 subunit EifCb)

1.33

0.64

-0.10

0.43

Yes

AFUA_1G06390 (translation elongation factor EF-1 alpha subunit)

2.00

1.28

-0.51

0.69

Yes

AFUA_2G13530 (translation elongation factor EF-2 subunit)

1.88

1.48

-0.43

0.62

Yes

AFUA_3G08160 (eukaryotic translation initiation factor 4)

1.81

1.48

0.84

1.18

Yes

AFUA_4G12920 (histidyl-tRNA synthetase, mitochondrial precursor)

1.10

1.20

0.64

1.18

Yes

AFUA_5G05920 (glycyl-tRNA synthetase)

1.56

1.26

0.23

0.83

Yes

AFUA_6G04570 (translation elongation factor eEF-1 subunit gamma)

1.20

1.10

0.22

0.71

Yes

AFUA_7G05660 (translation elongation factor eEF-3)

2.02

1.43

-0.19

0.70

Yes

AFUA_8G03880 (alanyl-tRNA synthetase)

1.37

0.80

-0.22

0.32

Yes

Transcription

AFUA_1G15620 (DEAD box RNA helicase HelA)

1.43

1.40

0.24

0.70

Yes

Cell cycle

AFUA_2G17110 (cell division control protein Cdc48)

1.01

1.11

0.50

0.94

Yes

Down regulated

Chaperone

AFUA_2G13040 (mitochondrial co-chaperone GrpE)

-1.12

-0.73

0.21

-0.57

Yes

Transport

AFUA_5G03690 (CRAL/TRIO domain protein)

-1.29

-0.61

0.37

-0.53

Yes

Energy generation

AFUA_2G13010 (cytochrome c oxidase polypeptide vib)

-1.79

-1.54

-0.12

-1.12

Yes

Carbohydrate metabolism

AFUA_1G09930 (glycerol dehydrogenase Gcy1)

-1.16

-1.44

-1.25

-0.98

No

AFUA_3G09230 (carboxylesterase)

0.14

-0.69

-1.07

-1.07

No

Nitrogen metabolism

AFUA_3G09320 (serine hydroxymethyltransferase)

0.06

-0.67

-1.01

-1.12

No

Protein biosynthesis/Translation

AFUA_6G02750 (nascent polypeptide-associated complex (NAC) subunit)

-1.35

-1.21

-0.85

-1.36

No

AFUA_6G12660 (40S ribosomal protein S10b)

-1.35

-1.21

-0.85

-1.36

No

Transcription

AFUA_3G08580 (glycine-rich RNA-binding protein)

-1.27

-1.18

-0.84

-2.09

No

AFUA_8G05300 (RNA polymerase II subunit 7)

-1.38

-0.97

-0.72

-1.81

No