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Figure 4 | BMC Genomics

Figure 4

From: A deep investigation into the adipogenesis mechanism: Profile of microRNAs regulating adipogenesis by modulating the canonical Wnt/β-catenin signaling pathway

Figure 4

Mir-210 represses WNT signaling through targeting Tcf7l2 and promotes adipogenesis. (A) MDI induction for 3 days in 3T3-L1 cells; (B) Mir-210 mimics were transfected in 3T3-L1 cells and following 3 days' MDI induction; (C) Low amplification of 3T3-L1 cells with or without miR-210 transfection following 3 days' MDI induction; (D) Relative expression of Pparg and (E) aP2 after transfection of miR-210 mimics and inhibitor; (F) Mir-210 blocked the expression of Tcf7l2 at transcription level in dose-dependent manner in 3T3-L1 cells; (G) Mir-210 binding site in 3'UTR of Tcf7l2 (up) and the synthesized sequence of 3'UTR of Tcf7l2 with Xho1 and Not1 emzyme sites (down). (H) Mir-210 inhibits renilla luciferase reporter activity with 3' UTR binding site derived from Tcf7l2 in HEK293FT cells; (I) Transfection of miR-210 increased the protein of β-catenin in cytoloplasm and (J) nucleus in 3T3-L1 cells.

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