Figure 3
Effect of masking probes over a range of signal intensity thresholds (0-640) on the number of probe sets commonly-selected in M. truncatula and alfalfa (―Δ―) and the correlation of the PES/ES hybridization intensity ratio for the commonly-selected genes (―O―). A signal intensity threshold of 40 (red star) was selected to mask biased probes. Commonly-selected genes are defined as genes exhibiting at least a 2-fold difference in hybridization intensity expression ratio between PES and ES internodes (PES vs. ES, ≥ 2-fold difference) of both alfalfa and M. truncatula.