Figure 5

Reverse Northern hybridization. A, Flowchart of the Reverse Northern analysis. B, cDNA clones enriched in EPSmax13 were spotted via slot blot technique on two nylon membranes and then hybridized with total cDNAs derived either from EPSmax13 (upper panel) or EPSmin17 (lower panel). C, as reference, the same membranes were hybridized with vector-DNA to normalize probe intensities. Differentially expressed genes are marked by a red frame.