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Table 3 Unigenes identified via SSH and Reverse Northern hybridization that display increased expression in EPSmax13 medium.

From: Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii

Target ID

Predicted function (TBLASTx)

Length (bp)*

Homologous 454 unigene

Carbohydrate metabolism

  

12 VII-3

Glucan phosphorylase

421

contig00741

   

contig15192

B5

UTP-glucose-1-phosphate uridylyltransferase

429

contig14249

F4

UDP-glucose-4-epimerase

531

contig14591

   

contig16714

   

contig05705

   

contig19066

D1

Glucosamine-6-phosphate isomerase

441

contig18828

   

contig19082

E3

Beta-fructofuranosidase

419

contig14026

   

contig07977

C2

Glycogen phosphorylase

470

contig13256

7 VI-14

Glycogen phosphorylase

421

contig00741

   

contig15192

3 VI-7

Isocitrate dehydrogenase

546

contig15308

   

contig19633

9 VI-19

Oxoglutarate dehydrogenase

325

No hit

33 XI-28

Pyruvate decarboxylase

318

contig19196

   

contig19387

E4

Pyruvate decarboxylase

242

No hit

G2

Pyruvate decarboxylase

236

contig19196

   

contig19387

G8

Pyruvate decarboxylase

242

contig03793

   

contig15593

D2

Phosphopyruvate hydratase

183

No hit

B8

Trehalose phosphorylase

178

contig17258

   

contig19103

F3

Mannitol-1-phosphate dehydrogenase

357

contig21872

   

contig13513

   

contig19371

4 VI-8

Formate dehydrogenase

186

contig08513

27 V-36

Formate dehydrogenase

486

contig04947

   

contig11312

   

contig16572

   

contig17132

   

contig13166

Lipid metabolism

  

29 X-12

Acetyl-CoA hydrolase/transferase

911

contig16913

   

contig18900

   

contig13924

34 XI-34

Oleate 12-hydroxylase gene

337

contig00730

   

contig19568

21 VIII-38

Multifunctional beta-oxidation protein

589

contig15214

   

contig15066

   

contig12394

8 VI-18

Acyl-CoA-Dehydrogenase

877

contig11819

   

contig00728

   

contig07196

Transport

  

13 VII-5

Endoplasmic reticulum-derived transport

451

contig14573

   

contig13652

A3

Copper transporter

481

contig15666

   

contig14830

   

contig02543

   

contig18312

Amino acid metabolism

  

22 VIII-45

Acetylornithine aminotransferase

146

No hit

6 VI-12

Acetylornithine aminotransferase

140

No hit

21 VIII-38

Aminotransferase

589

contig15214

   

contig15066

   

contig12394

A2

Aspartate aminotransferase

476

No hit

B7

Aspartate aminotransferase

538

contig16197

   

contig17262

   

contig08150

Oxidative stress

  

D4

Manganese superoxide dismutase

229

contig14464

Others

  

D3

Superfamily of calcium sensors and calcium signal modulators

351

contig18978

   

contig15800

   

contig15801

   

contig16990

18 VII-50

ATP synthase vacuolar proton pump

358

contig05644

   

contig12704

20 VIII-21

GAL4-like DNA-binding domain

340

contig04517

31 X-30

Plasma membrane H+ transporting ATPase

348

contig14871

B4

Intradiol dioxygenase

570

No hit

Hypothetical

  

A4

hypothetical protein UM02463.1

352

contig16014

14 VII-6

XP_001828655.1 CC1G_10527

345

No hit

24 IV-17

XP_001873967.1

470

No hit

28 X-11

XP_001875220.1

624

contig16711

   

contig08447

5 VI-11

XP_001873416.1

392

contig01594

   

contig20088

   

contig21715

   

contig15050

B2

XP_001830146.1 CC1G_09306

540

contig20176

   

contig17567

   

contig17591

   

contig21673

15 VII-9

Transcription factor

352

No hit

B6

No hit

590

contig02865

   

contig11989

   

contig00561

A8

No hit

193

No hit

A7

No hit

193

No hit

1 VI-4

No hit

207

contig14768

   

contig20467

   

contig09075

11 VII-2

No hit

241

contig15360

   

contig15302

G7

No hit

537

contig21711

   

contig12931

   

contig16495

C6

No hit

602

contig02865

   

contig11989

   

contig00561

F8

No hit

367

contig16532

   

contig19793

   

contig01157

  1. * The DNA sequences of all SSH unigenes are given in Additional file 4.