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Table 3 Unigenes identified via SSH and Reverse Northern hybridization that display increased expression in EPSmax13 medium.

From: Transcriptome sequencing and comparative transcriptome analysis of the scleroglucan producer Sclerotium rolfsii

Target ID Predicted function (TBLASTx) Length (bp)* Homologous 454 unigene
Carbohydrate metabolism   
12 VII-3 Glucan phosphorylase 421 contig00741
    contig15192
B5 UTP-glucose-1-phosphate uridylyltransferase 429 contig14249
F4 UDP-glucose-4-epimerase 531 contig14591
    contig16714
    contig05705
    contig19066
D1 Glucosamine-6-phosphate isomerase 441 contig18828
    contig19082
E3 Beta-fructofuranosidase 419 contig14026
    contig07977
C2 Glycogen phosphorylase 470 contig13256
7 VI-14 Glycogen phosphorylase 421 contig00741
    contig15192
3 VI-7 Isocitrate dehydrogenase 546 contig15308
    contig19633
9 VI-19 Oxoglutarate dehydrogenase 325 No hit
33 XI-28 Pyruvate decarboxylase 318 contig19196
    contig19387
E4 Pyruvate decarboxylase 242 No hit
G2 Pyruvate decarboxylase 236 contig19196
    contig19387
G8 Pyruvate decarboxylase 242 contig03793
    contig15593
D2 Phosphopyruvate hydratase 183 No hit
B8 Trehalose phosphorylase 178 contig17258
    contig19103
F3 Mannitol-1-phosphate dehydrogenase 357 contig21872
    contig13513
    contig19371
4 VI-8 Formate dehydrogenase 186 contig08513
27 V-36 Formate dehydrogenase 486 contig04947
    contig11312
    contig16572
    contig17132
    contig13166
Lipid metabolism   
29 X-12 Acetyl-CoA hydrolase/transferase 911 contig16913
    contig18900
    contig13924
34 XI-34 Oleate 12-hydroxylase gene 337 contig00730
    contig19568
21 VIII-38 Multifunctional beta-oxidation protein 589 contig15214
    contig15066
    contig12394
8 VI-18 Acyl-CoA-Dehydrogenase 877 contig11819
    contig00728
    contig07196
Transport   
13 VII-5 Endoplasmic reticulum-derived transport 451 contig14573
    contig13652
A3 Copper transporter 481 contig15666
    contig14830
    contig02543
    contig18312
Amino acid metabolism   
22 VIII-45 Acetylornithine aminotransferase 146 No hit
6 VI-12 Acetylornithine aminotransferase 140 No hit
21 VIII-38 Aminotransferase 589 contig15214
    contig15066
    contig12394
A2 Aspartate aminotransferase 476 No hit
B7 Aspartate aminotransferase 538 contig16197
    contig17262
    contig08150
Oxidative stress   
D4 Manganese superoxide dismutase 229 contig14464
Others   
D3 Superfamily of calcium sensors and calcium signal modulators 351 contig18978
    contig15800
    contig15801
    contig16990
18 VII-50 ATP synthase vacuolar proton pump 358 contig05644
    contig12704
20 VIII-21 GAL4-like DNA-binding domain 340 contig04517
31 X-30 Plasma membrane H+ transporting ATPase 348 contig14871
B4 Intradiol dioxygenase 570 No hit
Hypothetical   
A4 hypothetical protein UM02463.1 352 contig16014
14 VII-6 XP_001828655.1 CC1G_10527 345 No hit
24 IV-17 XP_001873967.1 470 No hit
28 X-11 XP_001875220.1 624 contig16711
    contig08447
5 VI-11 XP_001873416.1 392 contig01594
    contig20088
    contig21715
    contig15050
B2 XP_001830146.1 CC1G_09306 540 contig20176
    contig17567
    contig17591
    contig21673
15 VII-9 Transcription factor 352 No hit
B6 No hit 590 contig02865
    contig11989
    contig00561
A8 No hit 193 No hit
A7 No hit 193 No hit
1 VI-4 No hit 207 contig14768
    contig20467
    contig09075
11 VII-2 No hit 241 contig15360
    contig15302
G7 No hit 537 contig21711
    contig12931
    contig16495
C6 No hit 602 contig02865
    contig11989
    contig00561
F8 No hit 367 contig16532
    contig19793
    contig01157
  1. * The DNA sequences of all SSH unigenes are given in Additional file 4.