Skip to main content

Table 3 Primers used for real-time quantitative PCR.

From: High gene expression of inflammatory markers and IL-17A correlates with severity of injection site reactions of Atlantic salmon vaccinated with oil-adjuvanted vaccines

Accession no.

Name

Direction

Sequence

T*A

P** size (bp)

CB510337

T cell receptor alpha chain (TCAC)

Fwd

GCCTGGCTACAGATTTCAGC

66

107

  

Rev

GGCAACCTGGCTGTAGTAGC

  

CA052383

Complement component C6

Fwd

TCCAACGTGCCACTCTCCTC

64

131

  

Rev

CCGAACAGGGCTTCTACACC

  

CA044561

L-plastin (Lymphocyte cytosolic protein 1)

Fwd

CATGCGAACACAGTCAGAACC

62

111

  

Rev

CAACGCCAAGTACGCTATCAC

  

CA059313

Annexin A5

Fwd

GAAACTTCAACGCCAACCAAG

60

115

  

Rev

TGTCTCTGGCTGTTGCTACG

  

CA062753

SH2 domain protein 1A

Fwd

GGGAGTAGTCTCTGCTGTCC

62

95

  

Rev

GGCTCTACTGCCTCTGTGTG

  

CB493440

Proteasome subunit alpha type 4

Fwd

GTTCTAACCAATGAGCTGAGG

60

89

  

Rev

AACGCTGTCACCAACTGCTC

  

CB511680

Lysozyme C

Fwd

CACCGACTATGGCATCTTCC

58

129

  

Rev

CTGACCGCCACTGTGATGTC

  

CA767935

Cathepsin D

Fwd

CAGGCTGGTAAGACCATCTGC

58

127

  

Rev

TGTTGTCACGGTCGAACACAG

  

DN048269

Complement C3a

Fwd

GAGGAAAGGTGAGCCAGATG

58

106

  

Rev

TGTGTGTGTCGTCAGCTTCG

  

CA043655

Interferon regulatory factor-1 (IRF-1)

Fwd

GATGGGACCTGAACAAGGATG

58

132

  

Rev

GAAGGGAGTTCATTGCACAGC

  

A064221

MHC-II alpha

Fwd

GAACACAGCAGGACCCACAC

58

134

  

Rev

TCTCCAGTCTGCCCTTCACC

  

CX984314

Leukocyte cell derived chemotaxin (LECT-2)

Fwd

GCGAGATGGTCAAGTTTGGTC

58

115

  

Rev

TGATGCTCACGGTTTCCTCTG

  

CK991004

Immunoglobulin heavy chain constant region (IGHC)

Fwd

AGATGGACGCTTGTGGATCTC

58

118

  

Rev

GGTCTGGAGCAATGGGACAG

  

CB488287

Secreted protein acidic and rich in cysteine precursor (SPARC)

Fwd

CCAGCAGGTCCAGGGAGTG

60

156

  

Rev

TGCGTATGAGGGACTGGCTG

  

CA055453

Glutathione peroxidase

Fwd

GCAATCAGTTCGGACATCAGG

60

131

  

Rev

GTCCTTCCCATTCACATCCAC

  

CA050443

C1q-like adipose specific protein

Fwd

GTGATGACATTTTTGAAGATCAGG

60

104

  

Rev

AATAAGGAGAGAATGAAGGTGATC

  

CB516930

CD209 antigen

Fwd

CCCATCTCCAATCCCCTTCC

60

119

  

Rev

CCTGCACAGCAAGGAACAGC

  

CA051187

Mannose-binding protein C precursor

Fwd

CAAGAGGGGCTTGGTGTTGG

60

107

  

Rev

TGTTGTCAACATTGAGCCATGC

  

CA039888

IgM heavy chain membrane bound form

Fwd

TCTGGGTTGCATTGCCACTG

60

121

  

Rev

GTAGCTTCCACTGGTTTGGAC

  

CB492684

Annexin A1

Fwd

AGGAAGGGAACAGACTGCTC

60

120

  

Rev

AATAGCCTTTGCCACATCCAC

  

CB503743

CC chemokine (CCL 19)

Fwd

CCATGTAGCAGCAAGCACAG

66

128

  

Rev

GGCAGCTATCCGACATCCTC

  

CB510333

Cd 63

Fwd

AACAGTCTGACACCCCATCTG

58

97

  

Rev

TGTGCCAGACTCCTGCTGTG

  

CA056108

C type Lectin receptor A (CTL)

Fwd

ATCCTGCACAGCAAGGAACAG

58

128

  

Rev

TTGTCCACCCATCTCCAATCC

  

CK990996

Metallothionine

Fwd

GGACAGCAGGGGCAGCAAC

58

128

  

Rev

GCGATCAAAAACTGGAACATGG

  

CA058146

Putative complement factor D

Fwd

GAATCCATCGGCTGTACGAAG

64

115

  

Rev

CCGTTGGTGTGTAATGGGATG

  

AY693393

CD8α

Fwd

CACTGAGAGAGACGGAAGACG

56

174

  

Rev

TTCAAAAACCTGCCATAAAGC

  

DQ867018

CD4

Fwd

GAGTACACCTGCGCTGTGGAAT

58

123

  

Rev

GGTTGACCTCCTGACCTACAAAGG

  

FJ263446

Interferon gamma (IFN-γ)

Fwd

CTAAAGAAGGACAACCGCAG

60

159

  

Rev

CACCGTTAGAGGGAGAAATG

  

AF088999

Inducible nitric oxide sythase (iNOS)

Fwd

GGAGAGCCTTCTGGTTG

60

116

  

Rev

ACCTTAACTTGTTCCTGAGATAC

  

EF165028

IL-10

Fwd

CGCTATGGACAGCATCCT

59

84

  

Rev

AAGTGGTTGTTCTGCGTT

  

BK001401

Arginase 1b

Fwd

CATTGGCTTGAGAGACGTGGAT

60

68

  

Rev

AGTAACCTTTGACACCCAGCAA

  
 

Arginase 1b (Probe)

 

6FAM-CAGAAGAGCACCATATCC

  

AF321836

Elongation factor-1

Fwd

GCTGTGCGTGACATGAGG

60

88

  

Rev

ACTTTGTGACCTTGCCGC

  

EU418015

GATA-3

Fwd

CCCAAGCGACGACTGTCT

60

61

  

Rev

TCGTTTGACAGTTTGCACATGATG

  
 

GATA-3 (Probe)

 

FAM-TTCCTGCCCGTCTTGC

  

BT048013

Granzyme A

Fwd

GACATCATGCTGCTGAAGTTG

60

81

  

Rev

TGCCACAGGGACAGGTAACG

  

BT059144

IL-6 receptor

Fwd

TCCCTCAGTGCTACCTCCTC

60

138

  

Rev

CCAGGTGTGGCTCTCTACTC

  

GW574233

IL-17A

Fwd

TGGTTGTGTGCTGTGTGTCTATGC

60

136

  

Rev

TTTCCCTCTGATTCCTCTGTGGG

  

BT058869

IL-17A receptor

Fwd

CAAGTGGAGGGCGATGTGTG

60

107

  

Rev

TCGGGCAGGAAGAGGTTGAG

  

EU082211

TGF-β

Fwd

AGTTGCCTTGTGATTGTGGGA

60

191

  

Rev

CTCTTCAGTAGTGGTTTGTCG

  
  1. * Annealing temperature; **Product