Figure 6
From: A deletion mutation in bovine SLC4A2 is associated with osteopetrosis in Red Angus cattle
SLC4A2 deletion mutation genotyping assay. Representative PCR amplicons for each deletion mutation genotype, separated by agarose gel electrophoresis, are shown. From left to right, the first lane represents a known osteopetrosis carrier individual, the second and third lanes represent normal individuals and the fourth and fifth lanes represent osteopetrosis-affected calves. As shown, both normal (475 bp) and mutant (330 bp) alleles are amplified from a heterozygous individual, whereas only the appropriate allele is amplified from homozygous normal and mutant individuals.