The similarity of the EGP encoding genes demonstrated by multiple sequences alignment indicating the similarity. The three EGP genes are all differentially expressed under the change of water temperature. The EST BG27_B08 is homologous to salivary gland peptide identified from the western black-legged tick (Ixodes pacificus). The other two probesets are all homologous to the blacklegged tick (Ixodes scapularis). The nucleotides were colored with black when more than 50% nucleotides in this locus were identical. The gap between the nucleotides were labeled as "-".