Figure 4

The qRT-PCR results demonstrated the relative expression levels of the gene during the byssiogenesis. The byssogenesis and non-byssogenesis samples were treated as described in materials and methods, and the non-byssogenesis sample was used as control with value 1. The amount of the transcripts of the gene was calculated by using 2^-ΔΔCt model. The comparisons of the gene expression levels between byssogenesis and non-byssogenesis and detached sample were made at 0, 12 hours, 1 day, 2 days, and 3 days post-treatment.