Figure 2

RT-qPCR experiment supporting expression array data. For RT-qPCR, the cDNAs made using 4 untreated biological replicates were pooled in equal amounts and used in triplicate per experiment as a reference. For each of the treatments (dystrophin knockdown (KD) or GL2 siRNA), the biological replicates from two treatment occasions (two biological replicates per each treatment occasion) were used. For each treatment occasion, the cDNAs from each biological replicate were mixed in equal amounts and used in triplicate. Relative gene expression was calculated by the ΔΔCT method. Y axis: the normalised ratio (fold change) of expression between the treated samples (Dystrophin KD or GL2 siRNA control) versus untreated samples. Error bars indicate mean +/- 1 SD.