Volcano plots show ANOVA results. A. Eight microarrays of female samples for germline-conditioned (GL+) vs -naive (GL-) soma. The y-axis shows log10-x p-values and the x-axis is log2 fold expression. Gene lists are derived from the Fs test in MAANOVA (see Methods) indicated by orange data points. B. Volcano plot of six hybridizations of male germline-conditioned vs -naive soma. Significance and fold expression are as in A. C. Northern blots on genotypically matched (tud1/CyO) germline-conditioned (GL+) and naïve (GL-) gonadectomized male and female flies. RP49 serves as a loading control.