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Figure 2 | BMC Genomics

Figure 2

From: Functional genomics of human bronchial epithelial cells directly interacting with conidia of Aspergillus fumigatus

Figure 2

Differential staining of extracellular and internalized conidia by anti- A. fumigatus antibody. GFP-expressing A. fumigatus conidia and 16HBE14o- cells were co-incubated for 6 hrs, fixed and then stained with DAPI, to label cell nuclei, and a polyclonal anti-A. fumigatus antibody, to label extracellular conidia, before visualization by confocal microscopy. One representative field is shown in the following channels: A) wavelength 495 nm for GFP (green); B) wavelength 594 nm for anti-A. fumigatus antibody (red); C) wavelength 405 nm for DAPI (blue); D) merged GFP, anti-A. fumigatus antibody and DAPI image. Conidia not labeled by the anti-A. fumigatus antibody, and therefore only visible in the green but not red channel, were considered to be internalized by 16HBE14o- cells, allowing quantification of internalization.

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