Figure 6

Microscopic visualization of negative and positive sorted samples to determine the accuracy of sorting. FAC-sorted negative (A, B) and positive (C, D) cell samples were visualized by differential interference contrast (DIC) and fluorescence microscopy to verify the accuracy of sorting based on fluorescence intensity signals. Shown for each sample are DIC images (A, C) and green fluorescence images (B, D). Individual 16HBE14o- cells are clearly visible in the DIC images, while GFP-expressing A. fumigatus conidia present as bright green spots in the green fluorescence channel. The majority of cells in the negative samples were free of conidia, while most cells in positive samples were associated with at least one conidia spore.