Prednisolone-induced differential gene expression in mouse liver carrying wild type or a dimerization-defective glucocorticoid receptor

Table 2 Prednisolone-regulated genes associated with 'gluconeogenesis' in literature.

Biological process: gluconeogenesis
Gene name	Symbol	Fc WT	Fc GR^dim*
Insulin-like growth factor binding protein 1	Igfbp1	9.7	3.6
Tyrosine aminotransferase	Tat	4.1	1.6
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3	Pfkfb3	3.9	2.3
Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha	Ppargc1a	3.2	3.2
CCAAT/enhancer binding protein (C/EBP), beta	Cebpb	3.1	1.6
Protein-tyrosine sulfotransferase 2	Tpst2	2.7	1.5
Solute carrier family 2 (facilitated glucose transporter), member 1	Slc2a1	2.6	1.9
Forkhead box O1	Foxo1	2.2	1.7
Serine dehydratase	Sds	2.1	1.5
Aminoadipate-semialdehyde synthase	Aass	2.1	1.3
Phosphoenolpyruvate carboxykinase 1, cytosolic	Pck1	1.9	1.3
Tryptophan 2,3-dioxygenase	Tdo2	1.5	1.2
Aconitase 2, mitochondrial	Aco2	1.4	1.3
Transketolase	Tkt	-1.2	-1.1
Protein kinase, AMP-activated, beta 1 non-catalytic subunit	Prkab1	-1.5	1
Adiponectin receptor 2	Adipor2	-1.6	-1.3
Purinergic receptor P2Y, G-protein coupled, 5	P2ry5	-1.6	-1.3
Pyruvate kinase liver and red blood cell	Pklr	-2.1	-1.4
CCAAT/enhancer binding protein (C/EBP), alpha	Cebpa	-2.3	-1.2
Sterol regulatory element binding factor 1	Srebf1	-3	-1.6
Insulin receptor substrate 1	Irs1	-4	-1.5

CoPub identified 21 prednisolone-regulated genes in wild type (WT) mice that are associated in literature with gluconeogenesis. The identified genes share at least 3 publications and have an R-scaled score [34] of at least 35 with keyword 'gluconeogenesis' in Medline abstracts. For each gene, the fold change (Fc) in prednisolone versus vehicle-treated WT mice (Fc WT) is given. Additionally, for each gene also the Fc in prednisolone versus vehicle-treated GR^dim mice (Fc GR^dim) is shown. *It should be noted that in GR^dim mice these genes did not meet the p-value cutoff of 0.01 for significance after correction for multiple testing, and are mentioned to illustrate a small effect of prednisolone on gene expression in GR^dim mice.

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