Prednisolone-induced differential gene expression in mouse liver carrying wild type or a dimerization-defective glucocorticoid receptor

Table 3 Prednisolone-regulated genes associated with 'cell cycle' in literature.

Biological process: cell cycle
Gene name	Symbol	Fc WT	Fc GR^dim*
Growth arrest and DNA-damage-inducible 45 gamma	Gadd45g	17.8	2.9
Dual specificity phosphatase 1	Dusp1	8.2	1
Cyclin-dependent kinase inhibitor 1A (P21)	Cdkn1a	8.1	1
Growth arrest and DNA-damage-inducible 45 beta	Gadd45b	5.1	1
Polo-like kinase 3 (Drosophila)	Plk3	4.5	1
Retinoblastoma binding protein 8	Rbbp8	4.5	1.3
Bcl2-like 1	Bcl2l1	3	1.1
Tensin 1	Tns1	2.6	1.2
Protein tyrosine phosphatase 4a1	Ptp4a1	2.3	1.1
Forkhead box O1	Foxo1	2.2	1.7
PAX interacting (with transcription-activation domain) protein 1	Paxip1	1.9	1
Forkhead box O3a	Foxo3a	1.9	-1.1
E1A binding protein p300	Ep300	1.8	1
NIMA (never in mitosis gene a)-related expressed kinase 7	Nek7	1.8	1.1
Cell division cycle 2-like 6 (CDK8-like)	Cdc2l6	1.8	1.2
Cell division cycle 40 homolog (yeast)	Cdc40	1.4	1
Cyclin D2	Ccnd2	-1.5	-1.2
NIMA (never in mitosis gene a)-related expressed kinase 6	Nek6	-1.7	-1.2
CDC28 protein kinase regulatory subunit 2	Cks2	-2.3	-1.3
TSC22 domain family, member 1	Tsc22d1	-4	-2.3

CoPub identified 65 prednisolone-regulated genes in wild type (WT) mice that are associated in literature with the cell cycle (top 20 shown). The identified genes share at least 3 publications and have an R-scaled score [34] of at least 35 with keyword 'cell cycle' in Medline abstracts. For each gene, the fold change (Fc) in prednisolone versus vehicle-treated WT mice (Fc WT) is given. Additionally, for each gene also the Fc in prednisolone versus vehicle-treated GR^dim mice (Fc GR^dim) is shown. *It should be noted that in GR^dim mice these genes did not meet the p-value cutoff of 0.01 for significance after correction for multiple testing, and are mentioned to illustrate a small effect of prednisolone on gene expression in GR^dim mice.

ISSN: 1471-2164