Figure 5

Superfamily tree of PepI/PepR/PepL and EstA members. Based on MSA of the concatenated sequences of the four structural core regions identified by the protein 3D structure alignment. Orthologous proteins are indicated by the same color. The PepR subgroup from LAB is shadowed in pink, and the PepL subgroup is shadowed in yellow. The bacterial phyla are indicated. Red dots indicate the experimentally characterized genes and red triangles indicate the protein 3D structures used for the analyses. The event of the substitution of catalytic residue aspartate by glutamate in the PepR subgroup is indicated in the tree.