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Table 4 Validation of microarray expression data by quantitative real-time PCR for 25 selected genes

From: Muscle transcriptomic profiles in pigs with divergent phenotypes for fatness traits

GENE   MICROARRAY qPCR Correlation
   n = 68(34H vs 34L) n = 20(10H vs 10L)   
   Ratio p-value Ratio p-value r* p-value
ACACA 1.42 0.003 2.23 0.047 0.77 0.0002
ADIPOR2 1.39 0.001 1.49 0.354 0.59 0.0108
AGPAT2 0.73 0.01 0.44 0.043 0.60 0.0099
APOE 1.61 0.001 5.24 0.017 0.73 0.0006
CEBPD 1.24 0.004 1.05 0.078 0.69 0.0016
CES3 2.1 0.000003 3.41 0.008 0.83 < 0.0001
DGAT2 2.68 0.001 1.91 0.048 0.83 < 0.0001
ELOVL6 3.04 0.005 3.31 0.042 0.88 < 0.0001
FABP4 1.32 0.0002 2.33 0.037 0.92 < 0.0001
FASN 3.09 0.0008 2.54 0.046 0.88 < 0.0001
IGFBP5 0.68 0.0007 0.50 0.025 0.83 < 0.0001
IRS2 1.31 0.001 4.1 0.012 0.53 0.0231
LIPE 4.21 0.000002 3.91 0.001 0.76 0.0002
LPIN1 1.23 0.008 1.68 0.005 0.68 0.0018
MEF2A 1.37 0.0002 1.68 0.037 0.63 0.0051
NPC1 1.4 0.00006 3.69 0.018 0.48 0.0437
PIK3C2A 1.54 0.009 2.98 0.021 0.68 0.0020
PIK3R1 0.72 0.01 0.70 0.020 0.48 0.0424
PPARD 1.28 0.008 1.57 0.021 0.53 0.0248
PPARG 1.06 0.005 2.31 0.041 0.69 0.0016
PPARGC1A 2.38 0.004 1.57 0.152 0.96 < 0.0001
RXRG 0.68 0.002 1.12 0.621 -0.26 0.2911
SCD 3.07 0.004 4.04 0.156 0.96 < 0.0001
TBC1D1 1.17 0.0002 0.98 0.552 -0.16 0.5287
VEGFA 1.26 0.009 1.69 0.032 0.48 0.0444
  1. Expression ratios and p-values are indicated for both assays and correlation coefficients between qPCR and microarray results are also shown.
  2. H = HIGH group; L = LOW group; Expression ratios between H and L groups; *correlation coefficients between qPCR and microarray results; in bold, genes whose difference in expression between groups has been validated by qPCR. Arrows up and down indicate gene overexpression and downregulation in the HIGH group, respectively.