Polyacrylamide gel electrophoresis. cDNA-AFLP PCR products generated using different selective (2+/2+) primer combinations for the 24 h time point. PCR fragments that are in the range of 100-400 bp have been visualized using two different types of the polyacrylamide gels (A) PCR fragments were separated using 6.5% polyacrylamide gel and NEN Model 4300 DNA Analyzer (B) fragments separated on 8% polyacrylamide that ran on a V16-2 gel system apparatus and stained with 10,000× dilution of SybrGold for 20 minutes. Lanes: (M) DNA marker; (C) control group; (1-8) denotes treated group plus the primer combination used to generate the PCR product. Each pair of a control sample and an experimental sample (e.g., C1, C2, C3) was generated by PCR amplification using different primer sets (1-8). Some of the differentially expressed PCR fragments are indicated by arrows.