qRT-PCR analysis of selected cDNA-AFLP transcripts (A-C) and transcripts not derived from cDNA-AFLP (D-H):. (A) Glycosyl Hydrolase, (B) Serine Protease, (C-D) storage proteins Hex 70b and Vg, and (E-H) components on the honey bee immune response pathways including anti-microbial peptides. Control group of samples are represented by blue bars, the experimental samples are represented by green bars. Samples were done in triplicate and normalized by dividing the amount (pg or fg as described in Materials and Methods) of the gene of interest by the amount for the housekeeping gene, actin. Results are reported as an average of the triplicates plus the standard deviation. Significant by ANOVA at P < 0.05(*), P < 0.01(**), and P < 0.001(***).