Table 1 List of primers and annealing temperatures (Tm)°C used in qRT-PCR, and NCBI accession numbers
From: Transcriptional responses in Honey Bee larvae infected with chalkbrood fungus
Gene and GenBank number | Tm°C | Forward Primers 5'-3' | Reverse Primers 5'-3' |
|---|---|---|---|
Abaecin [U15954] | 65°C | GGTAGTGATATTTATCTTCGC | TTGAGGCCATTTAATTTTCGG |
Defensin-1 [U15955] | 65°C | GTTGAGGATGAATTCGAGCC | TTAACCGAAACGTTTGTCCC |
Lysozyme-1 [XM_001120995] | 67°C | GGAGGCGAGGATTCTGACTCAATG | TGTTGCATATCCCTCCGCTGTG |
Hexamerin 70b [XP_392868] | 67°C | CCGCTCTTCAAATGTGGTCTAC | GATAGGTAAAAGGTTTGTGGTTC |
Vitellogenin [XM_392349] | 65°C | TTCTGATAAAGGCGTTGCTCAC | CTCGTCGTCGGTCGGAACTT |
Trypsin like Serine protease [XM_393882] | 65°C | TTGTTTACCGGCGAAAAATC | ATGTTCACGACCACATCCAA |
Glycosyl hydrolase 18 [XM_397146.3] | 67°C | GTGGTGGCAAACAAGCTGAT | CGCTGCAAAATTGTTCCACGA |
MyD88 [XM_396644] | 65°C | GAGAGGTCTTGCTCATTTATGC | TCTCAAGTTTATCCACCATTTCA |
Actin [AB023025] | 67°C | GAAATGGCAACTGCTGCATC | GAGATCCACATCTGTTGGAA |