Table 7 Real time qRT-PCR results for genes used to validate DGE data
Tag | Gene Symbol | Gene Name | DGE FC | RT-PCR FC | P |
|---|---|---|---|---|---|
gctgctctgcagtctga | ACADVL | Acyl-Coenzyme A dehydrogenase, very long chain | 1.65 | 1.72 | 0.014 |
acccgagagacagccga | ACTN3 | Actinin, alpha 3 | -1.97 | -1.41 | NS |
ccactaccctcttactc | CALM3 | Calmodulin 3 | -2.75 | -1.81 | 0.028 |
gaaaacagtagctaaag | DAG1 | Dystroglycan 1 | -2.81 | -1.27 | 0.021 |
ggactgtctttattttt | IGFBP5 | Insulin-like growth factor binding protein-5 | -4.35 | -3.18 | 0.023 |
gagtgcagcctttcacc | MRPS21 | 28 S ribosomal protein S21, mitochondrial | 9.47 | 6.03 | 0.013 |
tatgacagaaaagcaac | MSTN | Myostatin | -4.35 | -4.97 | 0.004 |
tgttgaagcgatgcagt | PER2 | Period homolog 2 | +inf | 1.88 | 0.003 |
tgttggtaagtagatcg | PER3 | Period homolog 3 (Drosophila) | 2.07 | 1.74 | 0.001 |
tggctgtatggggaggc | SLC25A29 | Solute carrier family 25 member 29 | 2.50 | 1.22 | 0.035 |
gatgaagctgggatgca | TNNT3 | Troponin T Type 3 | 2.93 | 0.94 | NS |