Figure 1

Examples of types of alternative splicing analysed. The boxes represent exons while the lines are introns. The bold lines beneath the exons indicate probes that detected significant expression changes for each example and the arrows represent primers used for qRT-PCR analyses. (a) Alternative promoter use can be found by an increase in the expression detection levels for a probe set for one promoter compared to the others. The change in promoter use of Elmo1 alters the 5' untranslated region (white boxes) as well as the N-terminus of the protein. (b) The same analyses can be applied for alternative polyadenylation sites, in this case Kif1b. (c) Alternative exons can be included or excluded in the mature mRNA, thus altering the coding sequence as in the Kif2a pre-mRNA.