Figure 3

Gene expression profile of selected newt-zebrafish homologues. Quantification of gene expression patterns by RT-PCR of potential newt-zebrafish homologues during newt heart regeneration in undamaged (day 0) and damaged newt hearts 4, 7, 14, and 21 days (day 4 to day 21) after injury (n = 3 for each time point). a) Expression of muscle creatin kinase, hsp90 alpha, and slc25a4. Potential zebrafish homologues showed a similar expression pattern although reduced mRNA levels were observed at 4 days to 14 days after injury. b) Expression of tubulin alpha 1, keratin 4 and tubulin beta. A delayed time course was apparent in regenerating newt hearts with increased levels only at 21 days after injury. c) Expression of collagen typeI alpha 1, similar to ferritin heavy chain, and similar to cathepsin K. Similar changes in expression levels were found both in newts and zebrafish at all time points after injury. Expression changes of more than two fold were statistically significant with the exception of tubulin 1 alpha at 21 days after injury (p < 0.05 by paired students t-test). Error bars are shown as ± STDEV.