Table 1 Quantity and quality of paired-end sequencing reads
From: Mobile element scanning (ME-Scan) by targeted high-throughput sequencing
| Â | Replication experiment | Pooling experiment | ||
|---|---|---|---|---|
Quality classification | # read pairs | % of total | # read pairs | % of total |
Total read pairs | 3,047,279 | 100 | 2,389,900 | 100 |
Both reads of high quality (not a, b or c, below) | 2,998,412 | 98.4 | 2,315,412 | 96.9 |
   (a) Either read is > 85% any one base | 1,370 | 0.0450 | 1,628 | 0.0681 |
   (b) Either read has > 2 'N' base calls | 4,345 | 0.143 | 3,073 | 0.13 |
   (c) Adapter sequence detected in either read | 43,192 | 1.42 | 69,827 | 2.9 |
Both reads high quality, index valid | 2,963,735 | 97.3 | 2,287,571 | 95.7 |
Junction read has Alu sequence; both reads high quality; index valid | 2,874,329 | 94.3 | 2,201,101 | 92.1 |
Supports an Alu insertion in the final results* | 2,458,549 | 80.7 | 1,753,750 | 73.4 |