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Table 2 Observed vs. Expected frequencies of indexes in pooled samples

From: Mobile element scanning (ME-Scan) by targeted high-throughput sequencing

Individual

Index

Read Pairs*

Expected %

Observed %

Replication

    

A1

ACCAT

1,485,980

50

50.1

A1

TATTC

1,477,755

50

49.9

Pooling

    

A

ACCAT

88,418

4

3.86

B

TATTC

82,049

4

3.59

C

GGTTA

88,895

4

3.89

D2

CGCTA

355,469

16

15.5

D2

TTGAT

1,672,740

72

73.1

  1. * Only read pairs passing all QC filters are counted.
  2. 1 Two aliquots of a DNA sample from this individual were processed in parallel and pooled for sequencing.
  3. 2 A DNA sample from this individual was sonicated, then divided into two aliquots for subsequent library construction steps.