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Table 2 Observed vs. Expected frequencies of indexes in pooled samples

From: Mobile element scanning (ME-Scan) by targeted high-throughput sequencing

Individual Index Read Pairs* Expected % Observed %
Replication     
A1 ACCAT 1,485,980 50 50.1
A1 TATTC 1,477,755 50 49.9
Pooling     
A ACCAT 88,418 4 3.86
B TATTC 82,049 4 3.59
C GGTTA 88,895 4 3.89
D2 CGCTA 355,469 16 15.5
D2 TTGAT 1,672,740 72 73.1
  1. * Only read pairs passing all QC filters are counted.
  2. 1 Two aliquots of a DNA sample from this individual were processed in parallel and pooled for sequencing.
  3. 2 A DNA sample from this individual was sonicated, then divided into two aliquots for subsequent library construction steps.