Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

Table 1 Comparison of sequencing data from different cDNA libraries generated by standard TS oligo vs. iso₃TS oligo

cDNA synthesis method	Species	Avg length of read (nt)	Num. 454 reads (Mb)	Num. 2+ SMART oligos (concatamers)	% of concatamers
Standard TS oligo	Salvia divinorium	197	342263 (77.6)	70549	20.6
	Solanum lycopsericum	207	336745 (70.0)	60173	17.9
	Solanum habrochaites	165	329070 (54.4)	38231	11.6
Modified TS oligo (iso₃TS oligo)	Solanum habrochaites	199	344190 (68.6)	5032	1.5
	Isatis tinctoria	345	970893 (335.9)*	29734	3.1
	Equisetum hyemale	324	871579 (282.2)*	159	0.02

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