Skip to main content

Table 1 Comparison of sequencing data from different cDNA libraries generated by standard TS oligo vs. iso3TS oligo

From: Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

cDNA synthesis method Species Avg length of read (nt) Num. 454 reads (Mb) Num. 2+ SMART oligos (concatamers) % of concatamers
Standard TS oligo Salvia divinorium 197 342263 (77.6) 70549 20.6
  Solanum lycopsericum 207 336745 (70.0) 60173 17.9
  Solanum habrochaites 165 329070 (54.4) 38231 11.6
Modified TS oligo (iso3TS oligo) Solanum habrochaites 199 344190 (68.6) 5032 1.5
  Isatis tinctoria 345 970893 (335.9)* 29734 3.1
  Equisetum hyemale 324 871579 (282.2)* 159 0.02
  1. * data from 454 Titanium sequencing run