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Table 1 qPCR ratios of crosslinked-reversed versus non crosslinked DNAa

From: ChIP on Chip: surprising results are often artifacts

Crosslink reversionb uvrD (control) rpsD
37°C proteinase K 0.36 ± 0.07 0.03 ± 0.01
42/65°C proteinase K 0.59 ± 0.25 0.03 ± 0.01
65°C o. n. 0.47 ± 0.12 0.03 ± 0.01
100°C 10 min 1.03 ± 0.44 0.04 ± 0.02
  1. aRatios were calculated based on triplicate qPCR values for two independent cultures with the indicated standard deviation.
  2. bShort description of the crosslink reversion method. See method section for details.
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BMC Genomics

ISSN: 1471-2164

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