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Table 1 qPCR ratios of crosslinked-reversed versus non crosslinked DNAa

From: ChIP on Chip: surprising results are often artifacts

Crosslink reversionb

uvrD (control)

rpsD

37°C proteinase K

0.36 ± 0.07

0.03 ± 0.01

42/65°C proteinase K

0.59 ± 0.25

0.03 ± 0.01

65°C o. n.

0.47 ± 0.12

0.03 ± 0.01

100°C 10 min

1.03 ± 0.44

0.04 ± 0.02

  1. aRatios were calculated based on triplicate qPCR values for two independent cultures with the indicated standard deviation.
  2. bShort description of the crosslink reversion method. See method section for details.