Starvation alters the liver transcriptome of the innate immune response in Atlantic salmon (Salmo salar)

BMC Genomics

Table 6 Real time PCR analysis of genes selected for confirmation of microarray data.

Group:	SAA	TRL 5	Hepcidin	JunB	CAAT/EBP	COUP	C type lectin	Precerebellin
AFL vs PFL	31.7 ± 4.4*	17.2 ± 4.6*	100.0 ± 20.7*	18.1 ± 3.3*	2.5 ± 0.4	3.0 ± 1.5	3.6 ± 0.6*	15.9 ± 7.5*
ASL vs PSL	128.2 ± 43.8	60.5 ± 26.8*0	424.5 ± 66.2*	8.7 ± 1.4*	5.6 ± 3.0	0.9 ± 0.5*	4.3 ± 2.0*	27.1 ± 13.6*
PSL vs PFL	-6.6 ± 3.7	-1.0 ± 0.3	-1.5 ± 0.4	-0.6 ± 0.3	-1.0 ± 0.6	-13.4 ± 7.7	-1.1 ± 0.2	-0.5 ± 0.2*

Candidate gene expression in liver RNA isolated from Atlantic salmon fed or starved prior to bacterial infection. Values are fold increase (± sem). The groups are: AFL fish that were fed on normal diet prior to bacterial infection compared to fed uninfected fish, PFL. ASL: fish that were starved for 28 days prior to infection compared to uninfected starved fish PSL. PSL uninfected starved fish compared to uninfected fed fish, PFL. Gene expression was determined by real time PCR and normalized to the elongation factor 1α gene. Statistical analysis of expression data was by t-test, * indicates P < 0.05.

ISSN: 1471-2164