Starvation alters the liver transcriptome of the innate immune response in Atlantic salmon (Salmo salar)

BMC Genomics

Table 7 Primers for real time PCR analysis

Primer name	Gene name	Accession number	Primer sequence	Annealing temp	product size
Exs1F1	Hepcidin	AF542965.1	GCTGTTCCTTTCTCCGAGGTG	55°C	163
Exs1R1			GACAGCAGTTGCAGCACAAAC
sTRL5F1	TRL 5	AY628755.1	GACCCCGGTGTGGCTGAA	55°C	310
sTRL5R1			TGGCTGATTTGTTTTACGCTGT
Exs5F1	Jun B	CA056715.1	TACTGCACTGTTGGGACAGC	55°C	171
Exs5R1			GTTCAGTATGCCCCGAGTGT
Exst10F1	CAAT/EBP	DW550698	CAGCGGGTGTTAAGATCCAT	55°C	200
Exst10R1			GCAGCAGGAGGATCCAAGTA
Exs22F	COUP TF	CK887094.1	CATCGAAAGCCTGCAGGAGAAATC	55°C	165
Exs22R			CCTACCAAGCGGACGAAGAACAGC
Exs3F1	Precerebellin	CB511158.1	GAGGGAACTGACAGCCAGAG	55°C	246
Exs3R1			TTCCCAACATTGCAAGTGAA
Exs2F1	C-type lectin	CB516930	AATCAGTTTGGCAAGCAGCAGA	55°C	374
Exs2R1			AAGCGATTTGAGATGTTTTAGTG
Exs14F	SAA	BQ037050.1	CCCTGCAGGTGCTAAAGACAT	55°C	186
Exs14R			CCTCGACCACTGGAACCCTGAA
EF1AEF	ELF-1α	AF321836.1	CAAGGATATCCGTCGTGGCA	55°C	327
EF1aER			ACAGCGAAACGACCAAGAGG

PCR primers for real time PCR analysis to assay gene expression of cDNAs found to be differentially expressed by the diet and infection experiments. Amplified cDNA was monitored by Sybr green (BioRad) incorporation on an Opticon real time PCR machine.

ISSN: 1471-2164