Comparative analysis of secreted protein evolution using expressed sequence tags from four poplar leaf rusts (Melampsora spp.)

Table 1 Summary of Melampsora cDNA libraries characteristics

Species/Natural host	Material	Unisequences	Contigs	Singletons	Putative S^a (%)	Putative S+^b (%)
M. larici-populina/Populus nigra	Haustoria	1148	427	721	54 (4.7)	72 (6.3)
M. larici-populina/P. nigra	Ex planta	787	266	521	51 (6.5)	92 (11.7)
M. medusae f. sp. deltoidae/P. deltoides	Ex planta	937	452	485	95 (10.1)	131 (14.0)
M. medusae f. sp. tremuloidae/P. tremuloides	Ex planta	1547	571	976	98 (6.3)	246 (15.9)
M. occidentalis/P. trichocarpa	Ex planta	1625	493	1132	107 (6.6)	148 (9.1)

^aS: Initial predicted set of unisequences encoding putative secreted proteins.
^bS+: Final set of unisequences encoding putative secreted proteins, following reassignments based on reciprocal BLAST.

ISSN: 1471-2164