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Table 2 Identified protein spots and their accumulation pattern

From: Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE)

Spot No.a

Assignmentb

Species

Accesion No.c

pI (Th/Exp)

MW (Th/Exp)

Mowse scored

coverage %

E1 vs E2

E1 vs E3

E1 vs E4

E2 vs E3

E2 vs E4

E3 vs E4

N004

Low molecular weight heat shock protein

[Malus × domestica]

AAF34133

5.4/8.0

18.2/20.0

149

14.4

-1

1

0

1

1

-1

N005

Glyceraldehyde-3-phosphate dehydrogenase

[Nicotiana tabacum]

CAB39974

7.7/7.9

36.7/21.1

408

27.3

-1

-1

-1

1

1

0

N011

Low molecular weight heat shock protein

[Malus × domestica]

AAF34133

5.4/7.3

18.2/21.2

155

25.0

-1

0

-1

1

1

-1

N012

ADP-ribosylation factor B1C

[Arabidopsis thaliana]

NP_186962

7.7/7.2

21.7/21.2

50

13.0

1

-1

-1

-1

-1

0

N014

ADP-ribosylation factor 1

[Solanum tuberosum]

CAA52468

6.8/7.1

22.6/19.6

316

26.9

0

0

0

0

0

0

N021

PRU1_PRUAV Major allergen Pru av 1

[Prunus avium]

O24248

5.9/6.6

16.7/18.4

189

50.0

0

0

0

0

0

0

N023

Small heat shock protein

[Retama raetam]

AAL32036

5.8/6.7

17.9/20.9

133

16.5

-1

0

-1

1

1

-1

N026

Peroxiredoxin

[Populus tremula × Populus tremuloides]

AAL90751

5.6/6.5

17.4/20.4

107

21.0

0

0

0

0

0

0

N027

Low molecular weight heat shock protein

[Malus × domestica]

AAF34133

5.4/6.3

18.2/22.0

258

25.0

-1

-1

-1

1

1

0

N028

Eukaryotic translation initiation factor 4D

[Medicago sativa]

CAA42065

5.5/6.3

17.7/19.7

119

13.7

0

0

0

0

0

0

N029

Major allergen Pru p 1

[Prunus persica]

ABB78006

5.8/6.4

17.6/19.0

530

45.5

1

1

1

0

0

0

N031

Small heat shock protein

[Prunus persica]

AAR99375

6.0/6.3

17.4/18.5

286

50.6

-1

-1

-1

1

1

0

N032

Small heat shock protein

[Prunus persica]

AAR99375

6.0/6.3

17.4/18.5

71

24.7

-1

-1

-1

1

1

0

N033

Superoxide dismutase

[Nicotiana plumbaginifolia]

CAA39444

5.6/6.4

15.4/17

134

17.1

0

0

0

0

0

0

N035

Thioredoxin H

[Prunus persica]

AAL26915

6.0/5.6

18.1/14.5

234

38.2

-1

-1

-1

1

1

0

N037

Low molecular weight heat shock protein

[Malus × domestica]

AAF34133

5.4/6.0

18.2/20.0

155

13.8

-1

0

0

1

1

0

N042

NADH dehydrogenase subunit F

[Graffenrieda latifolia]

CAJ84510

7.8/7.7

28.7/28.0

91

6.7

-1

0

-1

1

1

-1

N053

Manganese superoxide dismutase

[Camellia sinensis]

AAT68778

7.8/6.9

25.6/27.4

222

13.5

0

0

0

0

0

0

N056

Putative quinone reductase

[Vitis vinifera]

AAO12869

5.6/6.6

17.6/26.8

111

9.8

0

0

0

0

0

0

N073

Pectin methylesterase

[Nicotiana tabacum]

CAB57457

9.9/8.1

29.3/36.3

68

4.5

-1

-1

0

-1

1

1

N077

Porin

[Prunus armeniaca]

AAD38145

7.1/7.4

29.7/34.2

174

9.8

1

0

1

-1

0

1

N083

Triose phosphate isomerase cytosolic isoform

[Solanum chacoense]

AAR11379

5.7/6.9

27.0/30.6

111

15.4

0

0

0

0

0

0

N084

Proteasome subunit alpha type 7

[Cicer arietinum]

Q9SXU1

6.9/6.8

27.0/31.6

115

14.5

0

0

0

0

0

0

N086

Cytosolic ascorbate peroxidase

[Fragaria × ananassa]

AAB95222

5.7/6.5

27.2/30.2

231

16.0

0

0

0

0

0

0

N093

Abscisic stress ripening-like protein

[Prunus persica]

AAL26889

5.7/6.2

20.8/31.1

142

34.7

0

0

1

-1

1

1

N096

Quinone-oxidoreductase QR2

[Triphysaria versicolor]

AAG53945

6.4/6.3

22.1/27.4

102

37.6

0

0

0

0

0

0

N111

Iron-binding protein

[Pyrus pyrifolia]

ABD66595

5.4/5.8

19.5/29.3

116

11.3

1

1

1

-1

-1

1

N138

Annexin

[Medicago sativa]

CAA52903

5.4/6.7

35.0/38.7

88

13.0

1

1

1

0

-1

0

N139

Annexin

[Medicago sativa]

CAA52903

5.4/6.9

35.0/38.8

74

10.7

0

0

0

0

0

0

N140

NAD-dependent malate dehydrogenase

[Prunus persica]

AAL11502

6.6/7.0

35.5/39.5

390

21.4

0

0

0

0

0

0

N162

1-aminocyclopropane-1-carboxylate oxidase

[Prunus persica]

CAA54449

5.2/5.5

36.2/43.7

279

35.0

0

-1

-1

1

0

-1

N163

1-aminocyclopropane-1-carboxylate oxidase

[Prunus persica]

CAA54449

5.2/5.6

36.2/43.7

363

28.0

-1

0

-1

1

1

-1

N164

1-aminocyclopropane-1-carboxylate oxidase

[Prunus persica]

CAA54449

5.2/5.7

36.2/43.7

778

53.3

-1

0

-1

1

1

-1

N165

Oxidoreductase

[Arabidopsis thaliana]

NP_173786

8.5/5.9

41.0/42.6

224

10.4

0

-1

-1

-1

-1

0

N170

Actin

[Helianthus annuus]

AAF82805

5.6/5.8

41.7/49.2

979

45.4

0

0

0

0

0

0

N172

Actin

[Helianthus annuus]

AAF82805

5.6/6.0

41.7/48.7

358

17.0

0

0

0

0

0

0

N183

Alpha-1,4-glucan-protein synthase [UDP-forming]

[Pisum sativum]

O04300

5.7/6.2

41.6/45.4

2002

40.1

0

0

-1

0

-1

-1

N187

Anthocyanidin synthase

[Prunus persica]

BAC98347

5.4/6.1

31.1/47.2

287

24.5

0

0

-1

0

-1

-1

N193

Endopolygalacturonase

[Prunus persica]

AAC64184

6.2/6.9

41.3/48.9

389

46.1

-1

0

-1

1

1

-1

N201

NADP-dependent isocitrate dehydrogenase

[Prunus persica]

AAL11503

6.5/7.0

46.6/49.1

372

12.3

-1

0

0

1

1

0

N208

Glutamate Dehydrogenase 1

[Arabidopsis thaliana]

NP_197318

6.4/7.2

44.5/47.9

147

8.8

0

-1

-1

-1

-1

0

N209

Phosphoserine aminotransferase, chloroplast precursor

[Spinacia oleracea]

P52877

8.3/7.7

47.2/47.6

231

11.2

0

0

-1

0

-1

-1

N222

Quinone-oxidoreductase QR1

[Triphysaria versicolor]

AAG53944

9.4/8.2

34.9/46.1

158

9.4

1

1

1

0

0

0

N228

Catalase

[Prunus persica]

CAD42909

7.0/7.6

57.1/57.9

1356

51.8

1

1

1

0

1

1

N229

Catalase

[Prunus persica]

CAD42909

7.0/7.6

57.1/58.0

1151

40.4

1

1

1

0

1

1

N231

Dehydrin-like protein

[Prunus persica]

CAC00637

6.5/7.4

48.0/61.7

540

24.5

-1

-1

-1

-1

-1

1

N232

Dehydrin-like protein

[Prunus persica]

CAC00637

6.5/7.4

48.0/61.7

681

24.1

0

-1

-1

-1

-1

0

N246

UTP-glucose-1-phosphate uridylyltransferase

[Pyrus pyrifolia]

O64459

6.0/6.5

51.8/55.7

522

34.4

0

0

0

0

0

0

N251

ATPase subunit

[Beta vulgaris subsp. vulgaris]

CAA48650

5.7/6.5

55.0/54.8

645

30.6

0

0

0

0

0

0

N255

Pyruvate decarboxylase

[Fragaria × ananassa]

AAL37492

6.0/6.9

65.3/71.3

70

6.8

-1

-1

-1

1

-1

-1

N303

NADP-dependent malic enzyme

[Vitis vinifera]

P51615

6.1/6.4

65.2/65.4

600

16.4

0

0

0

0

0

0

N304

NADP-dependent malic enzyme

[Vitis vinifera]

P51615

6.1/6.3

65.2/65.6

291

6.3

1

1

1

-1

0

1

N305

Pyruvate decarboxylase

[Fragaria × ananassa]

AAL37492

6.0/6.3

65.3/61.8

473

18.5

-1

0

-1

1

-1

-1

N310

Thaumatin-like protein

[Prunus persica]

AAM00216

8.3/7.0

25.8/33.2

99

8.5

0

0

-1

0

-1

-1

N313

Glutathione S-transferase

[Cucurbita maxima]

BAC21261

7.7/6.1

25.0/28.3

102

6.0

1

1

1

-1

-1

0

N322

Putative glycine-rich RNA binding protein 1

[Catharanthus roseus]

AAF31402

8.7/6.1

14.2/16.4

40

11.0

-1

-1

-1

-1

-1

0

N323

Hypothetical protein

[Oryza sativa (japonica cultivar-group)]

BAD87001

11.6/6.1

17.4/15.7

46

4.5

-1

-1

-1

-1

-1

0

  1. a: Spot numbers as assigned in Figure 3.
  2. b: Protein assignment based on LC-MS/MS identification.
  3. c: Gene bank accession number.
  4. d: Probability that the match is random. Only proteins with scores greater than the minimum value for MS-based identification confidence are reported. Additional data about MS are reported in the additional file 2.
  5. The values in the columns 9 to 14 indicate if the protein accumulation pattern changes between the conditions shown above them. Positive values indicate a higher abundance in the first condition whereas negative values indicate the opposite situation.