Figure 4
From: Deep sequencing discovery of novel and conserved microRNAs in trifoliate orange (Citrus trifoliata)
Expression patterns of novel miRNA predicted from C. trifoliata. qRT-PCR of Low Molecular Weight RNA isolated from tissues at different development stages. Lanes: r, root; s1, young stem; s2, old stem; l1, leaf1 (0.2 cm in diameter); l2, leaf2 (0.5 cm in diameter); l3, leaf3 (1 cm in diameter); fl1, flower bud; fl2, partly open flower; fl3, full open flower; fr1, fruit 1 (about 0.5 cm in diameter); fr2, fruit 2 (about 1.5 cm in diameter); and fr3, fruit 3 (about 2 cm in diameter). Each reaction was repeated three times and the template amount was corrected by 5.8 s rRNAs.