Mismatches between Uni-Minibar primers and vertebrate sequences. The histograms show the distribution of mismatch counts between (A) direct Uni-Minibar primer or (B) reverse Uni-Minibar primer and their target loci on mitochondrial DNA, as revealed by a ecoPCR run using Uni-Minibar primers to amplify our mitochondrial reference database. For this run, 8 mismatches were tolerated for each primer. The red curve and the associated right axis represent the cumulative fraction of amplified sequences with less that m mismatch. We can observe than a few species present a small count of mismatches; these sequences with a few mismatches are expected to be advantageously amplified in a DNA mix containing multiple species.