Table 1 Vertebrate primer pairs tested.
From: An In silico approach for the evaluation of DNA barcodes
Barcode name | Primer Name | Sequence | Fragment size * | Developed for | Reference |
|---|---|---|---|---|---|
COI | |||||
COI-1 | FF2d | TTCTCCACCAACCACAARGAYATYGG | 655 | Fish | [18] |
FR1d | CACCTCAGGGTGTCCGAARAAYCARAA | ||||
COI-2H | LCO1490 | GGTCAACAAATCATAAAGATATTGG | 658 | mainly Arthropods | [1] |
HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | ||||
COI-2 | C_VF1LFt1 | WYTCAACCAAYCANAANGANATNGG | 658 | Fish | |
C_VR1LRt1 | TARACTTCTGGRTGNCCNAANAANCA | ||||
COI-3 | C_FishF1t1 | TCRACYAAYCAYAAAGAYATYGGCAC | 652 | Fish | [18] |
C_FishR1t1 | ACYTCAGGGTGWCCGAARAAYCARAA | ||||
Uni-Minibar | UniMinibarR1 | GAAAATCATAATGAAGGCATGAGC | 130 | Eukaryota | [20] |
UniMinibarF1 | TCCACTAATCACAARGATATTGGTAC | ||||
Cyt- b | |||||
MCB | mcb398 | TACCATGAGGACAAATATCATTCTG | 472 | All Vertebrates | [30] |
mcb869 | CCTCCTAGTTTGTTAGGGATTGATCG | ||||
cytM | L14841 | CCATCCAACATCTCAGCATGATGAAA | 359 | All Vertebrates | |
H15149 | CCCCTCAGAATGATATTTGTCCTCA | ||||
16S | |||||
16Sr | 16Sar | CGCCTGTTTATCAAAAACAT | 573 | Mammals | |
16Sbr | CCGGTCTGAACTCAGATCACGT | ||||
16Sr2 | 16Sa2 | CGCCTGTTTACCAAAAACAT | 573 | All Vertebrates | this study, modif. from [28] |
16Sb | CCGGTCTGAACTCAGATCACGT | ||||
16Smam | 16Smam1 | CGGTTGGGGTGACCTCGGA | 140 | Mammals, ancient DNA | [21] |
16Smam2 | GCTGTTATCCCTAGGGTAACT |