An In silico approach for the evaluation of DNA barcodes

BMC Genomics

Table 2 Taxonomic coverage and resolution capacity (B_S) of the different barcodes tested.

	all vertebrates		Chondrichthyes		Actinopterigii		Amphibia		Sauropsida		Mammalia
	Taxonomic coverage
COI-1	0.03		0.00		0.03		0.01		0.02		0.04
COI-2H	0.01		0.00		0.00		0.06		0.00		0.00
COI-2	0.95		0.67		0.98		0.91		0.93		0.96
COI-3	0.45		0.67		0.49		0.41		0.53		0.32
Uni-Minibar	0.00		0.00		0.00		0.00		0.00		0.00
MCB	0.09		0.00		0.05		0.03		0.14		0.18
cytM	0.10		0.00		0.09		0.03		0.11		0.17
16Sr	0.90		0.50		0.94		0.94		0.64		0.98
16Sr2	0.93		0.50		0.94		0.94		0.86		0.98
16Smam	0.40		0.00		0.25		0.32		0.05		0.96
	Resolution capacity
	B_S	N	B_S	N	B_S	N	B_S	N	B_S	N	B_S	N
COI-1	1.00	49	*	-	1.00	16	*	-	1.00	11	*	-
COI-2	0.97	2113	*	-	0.96	538	1.00	76	0.97	311	0.98	235
COI-3	0.96	650	*	-	0.94	326	1.00	33	0.96	159	1.00	75
MCB	0.95	1426	*	-	0.88	203	*	-	0.95	841	0.97	364
cytM	0.90	935	*	-	0.80	177	*	-	0.99	272	0.94	476
16Sr	0.98	1730	*	-	0.97	624	1.00	118	0.99	243	0.99	560
16Sr2	0.98	1769	*	-	0.97	624	1.00	118	0.99	286	0.99	560
16Smam	0.83	3242	*	-	0.83	518	0.76	1297	0.90	351	0.90	1063

In the analysis of Resolutions, only primers amplifying more than 10 species per taxon are considered.
N: number of sequences amplified from Genbank.
* The resolution was not calculated as the primer pairs amplified 10 or less different species for this taxon.

ISSN: 1471-2164