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Table 5 Primer sequences (5' to 3') for the 14 qRT-PCR validation assays including 18 S, actin and eukaryotic elongation factor [TC11706] housekeeping primer sets utilised for normalisation

From: Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle

 

Anneal Temp° C

Forward

Reverse

a 18 S AFO-18656-363

60

CCTGAGAAACGGCTACCACATC

GTGCCGGGAGTGGGTAATT

a TC11706

60

CAAAGGACTCAAGGACTCTCTGC

ACGAGAACTGGTCAGTAAAGAAGC

a Actin

60

GACATCAAGGAGAAGCTYTGC

CGTTGCCGATGGTGATS

CK173243

60

ACGAGTTGACTGATTTGAATGTGG

CAGGGAGGTACTGCTAGTGTCG

TC5847

60

CAGCATTTAGTGGTATCCGTAGC

GTGGATACGGAGGAGGATATGG

CK174565

60

AGGGATACAACCACAACTCAGG

CGAAAGAAGACTGTCCGAATCC

TC9363

60

CATTTTCTGACGATAATTGCTACG

CAGCGTACTCATTGAACTTCTCC

TC9228

60

CTACTAGCTCTCGCCTTTCTCG

CAGTAAGTCTCGTACGGGAAGC

TC13140

60

CACTTTAGTTCGCAGTCTGTGG

GTTCACGATAGAGACATGAAGACG

TC5872

60

AGGGATACAACCACAACTCAGG

CGAAAGAAGACTGTCCGAATCC

TC9672

60

CGGTTCTGAGAAATTATTGAAGC

ATCTATAGTGACTGCACCACTTGC

TC5931

60

TTGTCTATCTTGAACTTGGGAAGG

CTGTCAAACAAGTTCTGACTATCG

TC6326

60

ACAGTCCTCTTTTGGAATGACG

CACTTTGGAGCATATCTGTAATGG

CK184446

50

TCAGCTTGTCTATCTTGAACTTGG

CTGTCAAACAAGTTCTGACTATCG

TC12051

60

AGGCCTATTACATGATGCTCTACC

TGTCAATTTATTGAGTTGCGTAGC

TC9814

60

TAAAGGCTGGAGTATCCGAATG

GCAAGTTTGTCAGGATTGTACG

TC8147

50

GAATTTCAGAGAGGAATCAAGTGC

GAACAGTTTCTTTCACGGAACC

  1. aHousekeeping genes utilised for normalisation of qRT-PCR