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Table 3 Comparison of fold changes between microarray and qRT-PCR

From: Transcriptional profiling of host gene expression in chicken embryo lung cells infected with laryngotracheitis virus

Gene Accession # Gene Symbol 1 Day 3 Day 5 Day 7 Day
   Micro-array RT-PCR Micro-array RT-PCR Micro-array RT-PCR Micro-array RT-PCR
AJ721110 VNN2 0.5 0.3 3.3 3.5 6.5 8.7 2.5 2.6
AF070478 MMP13 0.7 0.8 3.4 3.7 2.9 3.8 0.7 0.5
AF062392 MMP27 0.8 0.8 4.1 7.8 6.6 23.7 3.5 7.9
X03509 CKB 0.9 1.4 4.7 6.8 5.5 11.0 2.0 3.3
AJ309540 IL6 0.9 1.1 4.8 7.7 7.2 37.1 3.1 5.7
AF432506 FABP4 1.0 1.2 5.3 7.8 7.1 33.4 15.0 10.0
X65459 FABP7 1.1 1.5 0.3 0.8 0.8 1.1 0.2 0.3
AJ851480 ACSL1 1.2 0.9 7.3 6.0 8.7 8.0 7.3 7.6
AJ720861 LSS 1.3 1.1 1.1 0.7 1.3 1.1 0.2 0.1
AJ719295 Insulin induced gene 1 1.5 1.3 1.0 0.7 0.9 0.9 0.2 0.1
AJ719718 SC4MOL 1.1 1.7 0.8 0.9 1.1 1.3 0.2 0.2
U09350 VIP 2.0 2.4 0.2 0.1 0.3 0.2 0.2 0.2
U62026 CENPF 3.3 9.9 0.7 0.7 1.4 2.2 0.6 0.6
M16199 IL8 3.7 3.0 22.4 43.4 26.8 172.7 18.5 39.0
X02009 LTF 0.7 0.5 2.7 2.3 3.6 5.2 3.2 4.1
AJ004940 HSPA8 0.9 1.1 0.5 0.4 0.6 0.5 2.7 3.2
AF411083 SFTPA1 1.7 2.5 0.4 0.7 0.9 0.9 0.4 0.3
X16881 CDC2 1.9 2.2 0.4 0.3 0.7 0.6 0.4 0.4
U12438 RFC2 1.9 2.0 0.8 0.6 1.0 0.9 0.3 0.2
Y14971 CXC chemokine K60 3.7 7.9 17.3 60.3 19.7 206.4 11.3 38.2
  1. The gene expression levels of 20 genes at four different time points in microarray analysis were confirmed by qRT-PCR. The expression levels were presented by fold changes values in microarray analysis, while, for qRT-PCR, the values were calculated by 2-ΔΔCT method [31], which would be comparable to fold changes in the microarray. All values are mean values determined by the calculation from three replicating assays.