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Table 3 Comparison of fold changes between microarray and qRT-PCR

From: Transcriptional profiling of host gene expression in chicken embryo lung cells infected with laryngotracheitis virus

Gene Accession #

Gene Symbol

1 Day

3 Day

5 Day

7 Day

  

Micro-array

RT-PCR

Micro-array

RT-PCR

Micro-array

RT-PCR

Micro-array

RT-PCR

AJ721110

VNN2

0.5

0.3

3.3

3.5

6.5

8.7

2.5

2.6

AF070478

MMP13

0.7

0.8

3.4

3.7

2.9

3.8

0.7

0.5

AF062392

MMP27

0.8

0.8

4.1

7.8

6.6

23.7

3.5

7.9

X03509

CKB

0.9

1.4

4.7

6.8

5.5

11.0

2.0

3.3

AJ309540

IL6

0.9

1.1

4.8

7.7

7.2

37.1

3.1

5.7

AF432506

FABP4

1.0

1.2

5.3

7.8

7.1

33.4

15.0

10.0

X65459

FABP7

1.1

1.5

0.3

0.8

0.8

1.1

0.2

0.3

AJ851480

ACSL1

1.2

0.9

7.3

6.0

8.7

8.0

7.3

7.6

AJ720861

LSS

1.3

1.1

1.1

0.7

1.3

1.1

0.2

0.1

AJ719295

Insulin induced gene 1

1.5

1.3

1.0

0.7

0.9

0.9

0.2

0.1

AJ719718

SC4MOL

1.1

1.7

0.8

0.9

1.1

1.3

0.2

0.2

U09350

VIP

2.0

2.4

0.2

0.1

0.3

0.2

0.2

0.2

U62026

CENPF

3.3

9.9

0.7

0.7

1.4

2.2

0.6

0.6

M16199

IL8

3.7

3.0

22.4

43.4

26.8

172.7

18.5

39.0

X02009

LTF

0.7

0.5

2.7

2.3

3.6

5.2

3.2

4.1

AJ004940

HSPA8

0.9

1.1

0.5

0.4

0.6

0.5

2.7

3.2

AF411083

SFTPA1

1.7

2.5

0.4

0.7

0.9

0.9

0.4

0.3

X16881

CDC2

1.9

2.2

0.4

0.3

0.7

0.6

0.4

0.4

U12438

RFC2

1.9

2.0

0.8

0.6

1.0

0.9

0.3

0.2

Y14971

CXC chemokine K60

3.7

7.9

17.3

60.3

19.7

206.4

11.3

38.2

  1. The gene expression levels of 20 genes at four different time points in microarray analysis were confirmed by qRT-PCR. The expression levels were presented by fold changes values in microarray analysis, while, for qRT-PCR, the values were calculated by 2-ΔΔCT method [31], which would be comparable to fold changes in the microarray. All values are mean values determined by the calculation from three replicating assays.