Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse

BMC Genomics

Table 1 Candidate genes resulting from microarray studies comparing FVB/N Prnp^-/- versus FVB/N mice.

Genes	FVB/N Prnp-/-	FVB/N Prnp-/-	Protein
	Microarray	QPCR
	(Log2 ratio)	(ΔΔ CT)
Downregulated
Prnp	-1.63	-15	Prion protein or PrPc
Scg5	-1.37	-2.13	Neuroendocrine secretory protein 7B2
Upregulated
None

Differentially expressed genes detected by the microarray analysis are listed with the calculated differential ratio (log2) alongside the observed delta-delta CT resulting from the QPCR experiment. The comparative C_t method is known as the 2 ^{[delta][delta]Ct} method, where delta delta C_t = [delta]C_{t, sample} - [delta]C_{t, reference}

ISSN: 1471-2164