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Table 5 QPCR analysis of the expression of candidate genes resulting from microarray studies comparing Tg37xNFH-Cre versus Tg37 mice.

From: Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse

Genes Tg37xNFH-Cre Tg37xNFH-Cre  
  Microarray QPCR Protein
  (log2 ratio) (ΔΔ CT)  
10 weeks    
Glrx3 -0.6 0.34 glutaredoxin 3
Atp13a1 0.81 -0.08 ATPase type 13A1
Prnp Tg37 ND -5.62 Prion protein
14 weeks    
AV451297.1 -2.62 -0.02 Hypothetical protein
Ifitm3 -1.41 -0.12 Interferon-induced transmembrane protein 3
Erf1 -1.19 -1.12 Eukaryote class I release factor
Bace1 -0.48 -0.02 beta-site APP-cleaving enzyme 1
BB217622.2 0.81 0.64 Unknown
Riken D17 0.79 0.31 Unknown
Fgf2 0.48 0.47 fibroblast growth factor 2
Prnp Tg37 ND -6.43 Prion protein
  1. Differentially expressed genes detected by the microarray analysis are listed with the calculated differential ratio (log2) alongside the observed delta-delta CT resulting from the QPCR experiment. The comparative Ct method is known as the 2 - [delta][delta]Ct method, where delta delta Ct = [delta]Ct, sample - [delta]Ct, reference. Age of the analyzed mice is mentioned.