|
FVB/N PrP-/- mice
| |
|---|
|
Name
|
SEQUENCE (5' -3')
|
|---|
|
Prnp 5'
|
CAACCGAGCTGAAGCATTCTG
|
|
Prnp 3'
|
CGACATCAGTCCACATAGTC
|
|
scg5 5'
|
CCTTTATGAGAAAATGAAGGG
|
|
scg5 3'
|
GGACAGATTTCTTTGCCACA
|
|
Tg37xNFH-Cre mice
| |
|
Name
|
SEQUENCE (5' -3')
|
|
Ifitm3 5'
|
TCAGCATCCTGATGGTTGTT
|
|
Ifitm3 3'
|
TGTTACACCTGCGTGTAGGG
|
|
AV451297.1 5'
|
CCCGAAGCGTTTACTTTGAA
|
|
AV451297.1 3'
|
CCCTCTTAATCATGGCCTCA
|
|
Erf1 5'
|
TCGCTCCACCAACTAAGAAC
|
|
Erf1 3'
|
AAACACGGGAAACCTCACC
|
|
Prnp Tg37 5'
|
GAAGGAGTCCCAGGCCTATT
|
|
Prnp Tg37 3'
|
GCAGGAATGAGACACCACCT
|
|
Glrx3 5'
|
CATAAGCATGGTGTCCAAGG
|
|
Glrx3 3'
|
TGCCTTCTCTGCTTCGTAGA
|
|
Riken D17 5'
|
AAGCCTTCATAGCGAGTGGA
|
|
Riken D17 3'
|
TTCCAGACAAGTGGACCTGA
|
|
Bace1 5'
|
TCGACCACTCGCTATACACG
|
|
Bace1 3'
|
CTCCTTGCAGTCCATCTTGAG
|
|
Fgf2 B 5'
|
AGCGGCTCTACTGCAAGAAC
|
|
Fgf2 B 3'
|
GCCGTCCATCTTCCTTCATA
|
|
Atp13a1 5'
|
CGTGACAAGGGTGAAGATGG
|
|
Atp13a1 3'
|
ATAGTAAGAGAAGGCATTCC
|
|
BB217622.2 5'
|
CCAGTTCCGTCAAAGTACCC
|
|
BB217622.2 3'
|
CATGCAGATCTTCAGGTCCA
|
|
β-actin 5'
|
TGTTACCAACTGGGACGACA
|
|
β-actin 3'
|
GGGGTGTTGAAGGTCTCAAA
|
- The sequences of the oligonucleotides used in the QPCR experiments are listed; including those of the housekeeping gene that was used in the three described analyses. The sets of primers for the Erf1, Glrx3, BB217622.2 and the AV451297.1 loci were designed within a single exon. All the other sets were designed over exon-exon borders.
