Figure 1
From: Gene expression profiling in the lungs of pigs with different susceptibilities to Glässer's disease
Validation of H. parasuis infection status in 'Fully Resistant', 'Susceptible' and mock-inoculated 'Control' animals. RT-PCR using H. parasuis 16 S rRNA and pig β-actin primers on RNA from (A) mock-inoculated 'Control' animals, (B) 'Fully Resistant' animals and (C) 'Susceptible' animals. L denotes the DNA ladder lane. The number above each lane denotes the animal tested. For example lane 1 in panel A denotes animal C1; lane 5 in panel C denotes animal S5. The 16 S rRNA amplicon is 821 bp in size when either genomic DNA or cDNA is used as template. The β-actin amplicon is 182 bp in size when cDNA is used as template and 280 bp when genomic DNA is used. The sizes of molecular standards in the DNA ladder are indicated for comparison with amplicon sizes. PCR and RT-PCR controls for (D) 16 S rRNA and (E) β-actin reactions are also provided. The lower case letter above each lane denotes a specific control. For panel D: (a) RT-PCR on uninfected pig RNA. (b) No reverse transcriptase RT-PCR on uninfected pig DNA. (c) PCR on pig genomic DNA. (d) RT-PCR on H. parasuis RNA. (e) PCR on H. parasuis genomic DNA. (f) PCR negative control. For panel E: (a) RT-PCR on H. parasuis RNA. (b) PCR on H. parasuis genomic DNA. (c) RT-PCR on uninfected pig RNA. (d) PCR on pig genomic DNA. (e) No reverse transcriptase RT-PCR on uninfected pig RNA. (f) PCR negative control. Results shown are from one of two independent replicate RT-PCR experiments.