Figure 2

Comparison of relative expression differences for selected genes from the 'Fully Resistant' expression profiles determined by microarray and RT-qPCR. The relative expression fold change for genes identified as being differentially expressed in the (A) FR v C comparison at 24 hours, (B) FR v S comparison at 24 hours, (C) FR v C comparison at 72 hours, and (D) FR v S comparison at 72 hours. For each gene, the black bar indicates the fold change as determined by microarray; the grey bar indicates the expression ratio as determined by RT-qPCR, with associated standard error bars. Relative expression ratios are given as the ratio of the first group compared to the second (e.g. 'Fully Resistant' compared to 'Control' in the graph in panel A). Statistical significance of the relative expression ratio is indicated (* p < 0.05; ** p < 0.01). Results for TMSL3 at 72 hours were not plotted on graphs C and D because of the axis scale chosen. The array fold changes for this gene in the FR v C and FR v S comparisons were -5.26 and -4.85 whereas the RT-qPCR fold changes were -101.75 and -73.47 respectively, the last being statistically significant (p < 0.05). Primer information is provided in additional file 5.