Microarray Analysis of Response of Salmonella during Infection of HLA-B27- Transfected Human Macrophage-Like U937 Cells

Table 4 Primers used in the gene mutagenesis in this study

Genes	Primers	Sequences
ssaN	ssaN-Km-F	5'-CTTTGCTATCTCCTTTTACGAGTACAATCGGGCTTCACTGC
		GGGCAGCAAGTGATGGCCTAAAGCCACGTTGTGTCTCAA-3'^α
	ssaN-Km-R	5'-CTTCAGACAGTGTAAAATCGATGAATTCGCGGACTTCTCGT
		CCACGTTCACCAATTAACAGCGCTGAGGTCTGCCTCGTG-3'^α
	ssaN-F	5'-CGTTGTTAAATGCGTGGTTG-3'
	ssaN-R	5'-CCCATTCCCGTACGTTCTAA-3'
ssaR	ssaR-Km-F	5'-GCGCTTGTACTTTCCTTATTCATTATGGGGCCGACGCTATT
		AGCTGTAAAAGAGCGCTGGAAAGCCACGTTGTGTCTCAA-3'^α
	ssaR-Km-R	5'-ATTAATATGAGCAAAGAATCAGGTTTTATCTTTCTTTTTAT
		GTTCTTCAGGCCAGGTTCGGCGCTGAGGTCTGCCTCGTG-3'^α
	ssaR-F	5'-TATCGCACTGTATGGCCTTG-3'
	ssaR-R	5'-ACCGCCTGCCAGTAAAAATA-3'
sspH2	sspH2-Km-F	5'-CTTTATGAAGTTTTCCGTCTCACTCAGTCTGTCCAGGAAGA
		GGCTGAATGCGTCGGCGTTAAAGCCACGTTGTGTCTCAA-3'^α
	sspH2-Km-R	5'-ACACTGGTTATTCCTGATAATAATCTGACCAGCCTGCCGGC
		GCTGCCGCCAGAACTGCGGGCGCTGAGGTTGTGTCTCAA-3'^α
	sspH2-F	5'-TCATCTTCAGCCAGTTGTGC-3'
	sspH2-R	5'-GTAATCGCCGCATTTATCGT-3'

^α: 80-nucleotide (nt)-long primers including 60 nt homology extensions complementary to the targeted regions of the genes and 20 nt priming sequences (underlined) for the synthesis of the kanamycin resistance cassette gene from E. coli MC4100 ybeW::Km.

ISSN: 1471-2164