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Table 2 Activity of ALPs on phosphosubstrates.

From: A new family of phosphoinositide phosphatases in microorganisms: identification and biochemical analysis

 

Specific Activity (nmoles PO4/min/mg)

Substrate

MptpB

LM1

Lmo1935

Lmo 1800

pNPP

46.94 ± 0.31

45.47 ± 1.52

57.47 ± 0.96

63.38 ± 0.71

   5' AMP

2.89 ± 0.55

0.34 ± 0.26

0.48 ± 0.02

6.71 ± 0.43

   5' IMP

3.35 ± 0.16

N.D

0.35 ± 0.03

3.90 ± 0.66

   P-Choline

0.63 ± 0.09

N.D

0.26 ± 0.05

0.58 ± 0.16

   P-Ethanolamine

0.51 ± 0.23

N.D

0.2 ± 0.02

0.37 ± 0.14

   Glycerol 2-phosphate

0.95 ± 0.26

N.D

0.12 ± 0.03

1.07 ± 0.57

   P-Ser

1.0 ± 0.12

3.17 ± 1.36

0.57 ± 0.21

N.D

   P-Thr

0.69 ± 0.05

N.D

0.42 ± 0.04

0.78 ± 0.34

   P-Tyr

6.48 ± 0.40

N.D

0.54 ± .04

9.68 ± 3.29

   NaP

1.24 ± 0.07

N.D

0.45 ± 0.1

0.15 ± 0.05

  1. Dephosphorylation of pNPP is shown as a reference.
  2. N.D, no activity detected.