Comparative analysis of expressed sequence tags from three castes and two life stages of the termite Reticulitermes flavipes

Table 2 In silico analysis of transcript bias in contigs formed from all sequences in each library, the ten highest R-values were analyzed using BLASTX (e-value ≤1E^-10).

Library Bias	Contig #	Length (bp)	R¹	p-value²	Identity	e-value	Identity (%)
Soldier	3	2647	106	5.24^e-50	Viral Protein	1.00^e-170	44
Soldier	794	1435	42	4.51^e-25	Ejaculatory Bulb-specific Protein 3	1.00^e-25	67
Alate	827	1036	31	1.42^e-10	Ejaculatory Bulb-specific Protein 3	1.00^e-29	73
Alate	148	981	66	6.63^e-21	ATP Synthase Subunit A	1.00^e-27	68
Alate	168	1724	26	1.03^e-10	Cyctochrome C Oxidase Subunit 1	1.00^e-180	86
Alate	352	1550	22	1.58^e-11	Endoglucanase B Precursor (Cellulase B)	1.00^e-109	59
Early Larval	472	954	92	3.61^e-48	Cytochrome Oxidase Subunit 3	1.00^e-86	76
Early Larval	479	1740	74	2.82^e-35	Cytochrome B	1.00^e-134	77
Early Larval	507	735	40	3.24^e-20	Tropomyosin	1.00^e-18	89
Early Larval	821	1349	18	7.46^e-05	Troponin C	1.00^e-38	70
Late Larval	412	975	80	3.10^e-22	Ribosomal Protein L31	1.00^e-51	83

¹ R-statistic was based on equations in Stekel et al. (2000) and compared relative abundance of each libraries prevalence in contiguous sequences from all available sequences.
² p-values were calculated to establish appropriate significance thresholds to confirm individual library abundance. p-value is in reference to significance of caste bias of singletons in contigs formed using a chi-squared test on singleton representation in each contig.

ISSN: 1471-2164